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Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A.
Varrot A, Frandsen TP, Driguez H, Davies GJ. Varrot A, et al. Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2201-4. doi: 10.1107/s0907444902017006. Epub 2002 Nov 23. Acta Crystallogr D Biol Crystallogr. 2002. PMID: 12454501
Crystallization and preliminary X-ray analysis of a fungal endoglucanase I.
Davies G, Tolley S, Wilson K, Schülein M, Wöldike HF, Dodson G. Davies G, et al. J Mol Biol. 1992 Dec 5;228(3):970-2. doi: 10.1016/0022-2836(92)90879-o. J Mol Biol. 1992. PMID: 1469728
Characterization of Escherichia coli OtsA, a trehalose-6-phosphate synthase from glycosyltransferase family 20.
Gibson RP, Lloyd RM, Charnock SJ, Davies GJ. Gibson RP, et al. Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):349-51. doi: 10.1107/s090744490102056x. Epub 2002 Jan 24. Acta Crystallogr D Biol Crystallogr. 2002. PMID: 11807274
Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold.
Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Nurizzo D, et al. Nat Struct Biol. 2002 Sep;9(9):665-8. doi: 10.1038/nsb835. Nat Struct Biol. 2002. PMID: 12198486
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state.
Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, Davies GJ. Ducros VM, et al. Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. doi: 10.1002/1521-3773(20020802)41:15<2824::AID-ANIE2824>3.0.CO;2-G. Angew Chem Int Ed Engl. 2002. PMID: 12203498 No abstract available.
Convergent evolution sheds light on the anti-beta -elimination mechanism common to family 1 and 10 polysaccharide lyases.
Charnock SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ. Charnock SJ, et al. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12067-72. doi: 10.1073/pnas.182431199. Epub 2002 Sep 9. Proc Natl Acad Sci U S A. 2002. PMID: 12221284 Free PMC article.
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose.
Charnock SJ, Bolam DN, Nurizzo D, Szabó L, McKie VA, Gilbert HJ, Davies GJ. Charnock SJ, et al. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. doi: 10.1073/pnas.212516199. Epub 2002 Oct 21. Proc Natl Acad Sci U S A. 2002. PMID: 12391332 Free PMC article.
Direct experimental observation of the hydrogen-bonding network of a glycosidase along its reaction coordinate revealed by atomic resolution analyses of endoglucanase Cel5A.
Varrot A, Davies GJ. Varrot A, et al. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):447-52. doi: 10.1107/s0907444902023405. Epub 2003 Feb 21. Acta Crystallogr D Biol Crystallogr. 2003. PMID: 12595701
Previous medium-resolution analyses of a series of enzymatic snapshots has revealed conformational changes in the substrate along the reaction coordinate (Davies et al., 1998). ...
Previous medium-resolution analyses of a series of enzymatic snapshots has revealed conformational changes in the substrate along the reacti …
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