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Quantitation of Cryptosporidium parvum infection in cell culture using a colorimetric in situ hybridization assay.
Rochelle PA, Ferguson DM, Johnson AM, De Leon R. Rochelle PA, et al. Among authors: de leon r. J Eukaryot Microbiol. 2001 Sep-Oct;48(5):565-74. doi: 10.1111/j.1550-7408.2001.tb00192.x. J Eukaryot Microbiol. 2001. PMID: 11596921
Polymorphisms in the beta-tubulin gene of Cryptosporidium parvum differentiate between isolates based on animal host but not geographic origin.
Rochelle PA, Jutras EM, Atwill ER, De Leon R, Stewart MH. Rochelle PA, et al. Among authors: de leon r. J Parasitol. 1999 Oct;85(5):986-9. J Parasitol. 1999. PMID: 10577745
Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples.
Rochelle PA, De Leon R, Johnson A, Stewart MH, Wolfe RL. Rochelle PA, et al. Among authors: de leon r. Appl Environ Microbiol. 1999 Feb;65(2):841-5. doi: 10.1128/AEM.65.2.841-845.1999. Appl Environ Microbiol. 1999. PMID: 9925626 Free PMC article.
An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum.
Rochelle PA, Ferguson DM, Handojo TJ, De Leon R, Stewart MH, Wolfe RL. Rochelle PA, et al. Among authors: de leon r. Appl Environ Microbiol. 1997 May;63(5):2029-37. doi: 10.1128/AEM.63.5.2029-2037.1997. Appl Environ Microbiol. 1997. PMID: 9143132 Free PMC article.
Development of a rapid detection procedure for Cryptosporidium, using in vitro cell culture combined with PCR.
Rochelle PA, Ferguson DM, Handojo TJ, De Leon R, Stewart MH, Wolfe RL. Rochelle PA, et al. Among authors: de leon r. J Eukaryot Microbiol. 1996 Sep-Oct;43(5):72S. doi: 10.1111/j.1550-7408.1996.tb05003.x. J Eukaryot Microbiol. 1996. PMID: 8822868 No abstract available.
Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water.
Rochelle PA, De Leon R, Stewart MH, Wolfe RL. Rochelle PA, et al. Among authors: de leon r. Appl Environ Microbiol. 1997 Jan;63(1):106-14. doi: 10.1128/AEM.63.1.106-114.1997. Appl Environ Microbiol. 1997. PMID: 8979344 Free PMC article.
UV inactivation of Cryptosporidium hominis as measured in cell culture.
Johnson AM, Linden K, Ciociola KM, De Leon R, Widmer G, Rochelle PA. Johnson AM, et al. Among authors: de leon r. Appl Environ Microbiol. 2005 May;71(5):2800-2. doi: 10.1128/AEM.71.5.2800-2802.2005. Appl Environ Microbiol. 2005. PMID: 15870378 Free PMC article.
Genotyping Cryptosporidium parvum with an hsp70 single-nucleotide polymorphism microarray.
Straub TM, Daly DS, Wunshel S, Rochelle PA, DeLeon R, Chandler DP. Straub TM, et al. Appl Environ Microbiol. 2002 Apr;68(4):1817-26. doi: 10.1128/aem.68.4.1817-1826.2002. Appl Environ Microbiol. 2002. PMID: 11916701 Free PMC article.
Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum.
Rochelle PA, Marshall MM, Mead JR, Johnson AM, Korich DG, Rosen JS, De Leon R. Rochelle PA, et al. Among authors: de leon r. Appl Environ Microbiol. 2002 Aug;68(8):3809-17. doi: 10.1128/aem.68.8.3809-3817.2002. Appl Environ Microbiol. 2002. PMID: 12147476 Free PMC article.
There was a good correlation between the average infectivity for HCT-8 cells and the results for CD-1 mice across all isolates for untreated oocysts (r = 0.85, n = 25) and for oocysts exposed to ozone and UV light (r = 0.89, n = 29). ...
There was a good correlation between the average infectivity for HCT-8 cells and the results for CD-1 mice across all isolates for untreated …
Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR.
Schwab KJ, De Leon R, Sobsey MD. Schwab KJ, et al. Among authors: de leon r. Appl Environ Microbiol. 1995 Feb;61(2):531-7. doi: 10.1128/AEM.61.2.531-537.1995. Appl Environ Microbiol. 1995. PMID: 7574592 Free PMC article.
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