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A centriole-free Drosophila cell line. A high voltage EM study.
Szöllösi A, Ris H, Szöllösi D, Debec A. Szöllösi A, et al. Among authors: debec a. Eur J Cell Biol. 1986 Mar;40(1):100-4. Eur J Cell Biol. 1986. PMID: 3084259
A hypotonic treatment of the cells before fixation allowed a clear recognition of centrioles in 1 micron thick sections. Three different approaches were used to determine the presence of absence of centrioles in a Kc control cell line and in the 1182-4D cell
A hypotonic treatment of the cells before fixation allowed a clear recognition of centrioles in 1 micron thick sections. Three
Microtubule cytoskeleton remodeling by acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells.
Moutinho-Pereira S, Debec A, Maiato H. Moutinho-Pereira S, et al. Among authors: debec a. Mol Biol Cell. 2009 Jun;20(11):2796-808. doi: 10.1091/mbc.e09-01-0011. Epub 2009 Apr 15. Mol Biol Cell. 2009. PMID: 19369414 Free PMC article.
Cytoskeleton microtubules undergo a reversible metamorphosis as cells enter and exit mitosis to build a transient mitotic spindle required for chromosome segregation. Centrosomes play a dominant but dispensable role in microtubule (MT) organization throughout …
Cytoskeleton microtubules undergo a reversible metamorphosis as cells enter and exit mitosis to build a transient mitotic spin …
Drosophila centrosomes are unable to trigger parthenogenetic development of Xenopus eggs.
Tournier F, Bobinnec Y, Debec A, Santamaria P, Bornens M. Tournier F, et al. Among authors: debec a. Biol Cell. 1999 Mar;91(2):99-108. doi: 10.1016/s0248-4900(99)80034-3. Biol Cell. 1999. PMID: 10399825
We further show that Drosophila centrosomes which possess a typical centriole architecture, and are quite active to nucleate microtubules in Xenopus cytoplasmic extracts, are unable to trigger egg cleavage. ...We conclude that the parthenogenetic activity of a centr …
We further show that Drosophila centrosomes which possess a typical centriole architecture, and are quite active to nucleate microtub …
Microtubule polyglutamylation in Drosophila melanogaster brain and testis.
Bobinnec Y, Marcaillou C, Debec A. Bobinnec Y, et al. Among authors: debec a. Eur J Cell Biol. 1999 Sep;78(9):671-4. doi: 10.1016/S0171-9335(99)80053-3. Eur J Cell Biol. 1999. PMID: 10535310
The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated in Drosophila melanogaster using the specific monoclonal antibody GT335. ...Neuronal microtubules are glutamylated on alpha-tubulin only wher …
The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated i …
Live analysis of free centrosomes in normal and aphidicolin-treated Drosophila embryos.
Debec A, Kalpin RF, Daily DR, McCallum PD, Rothwell WF, Sullivan W. Debec A, et al. J Cell Biol. 1996 Jul;134(1):103-15. doi: 10.1083/jcb.134.1.103. J Cell Biol. 1996. PMID: 8698807 Free PMC article.
However, the later phase of separation observed for centrosomes associated with a spindle (anaphase B) does not occur. Free centrosome separation regularly occurs on a plane parallel to the plasma membrane. ...This indicates the presence of a feedback mechani …
However, the later phase of separation observed for centrosomes associated with a spindle (anaphase B) does not occur. Free centrosom …
Methods for the study of centrosomes in Drosophila during embryogenesis.
Tram U, Riggs B, Koyama C, Debec A, Sullivan W. Tram U, et al. Among authors: debec a. Methods Cell Biol. 2001;67:113-23. doi: 10.1016/s0091-679x(01)67008-0. Methods Cell Biol. 2001. PMID: 11550463 No abstract available.
Differential properties of the two Drosophila gamma-tubulin isotypes.
Raynaud-Messina B, Debec A, Tollon Y, Garès M, Wright M. Raynaud-Messina B, et al. Among authors: debec a. Eur J Cell Biol. 2001 Oct;80(10):643-9. doi: 10.1078/0171-9335-00195. Eur J Cell Biol. 2001. PMID: 11713868
In contrast, gamma-tub37CD is undetectable in the soluble fraction and exhibits a tight binding to the centrosome. Syncytial embryos also contain the two gamma-tubulin isotypes, which are differentially recruited at the centrosome. ...They suggest the direct implication of …
In contrast, gamma-tub37CD is undetectable in the soluble fraction and exhibits a tight binding to the centrosome. Syncytial embryos …
Evolution of karyotype in haploid cell lines of Drosophila melanogaster.
Debec A. Debec A. Exp Cell Res. 1984 Mar;151(1):236-46. doi: 10.1016/0014-4827(84)90371-9. Exp Cell Res. 1984. PMID: 6421610
Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were quickly overgrown by diploid cells. ...Crossing of mh 1182/mh 1182 females with males bearing a ring X chromosome shows that the haploi …
Karyotype analysis revealed a high level of aneuploid cells with frequent chromosome fragments. In three lines, haploid cells were qu …
The centrosome cycle in syncytial Drosophila embryos analyzed by energy filtering transmission electron microscopy.
Debec A, Marcaillou C, Bobinnec Y, Borot C. Debec A, et al. Biol Cell. 1999 May-Jun;91(4-5):379-91. Biol Cell. 1999. PMID: 10519001
These new filamentous structures are: 1) a very orderly lattice that connects the two centrioles during mitosis; and 2) a fibrogranular connection between the centrosome and the nuclear envelope. ...This leaves a surprisingly short time to assemble a d …
These new filamentous structures are: 1) a very orderly lattice that connects the two centrioles during mitosis; and 2) a fibr …
Structural alterations of the mitotic apparatus induced by the heat shock response in Drosophila cells.
Debec A, Marcaillou C. Debec A, et al. Biol Cell. 1997 Mar;89(1):67-78. doi: 10.1016/s0248-4900(99)80082-3. Biol Cell. 1997. PMID: 9297784
Its two main characteristics are a very polarized spindle and a strong compartmentalization, ensured by large remnants of the nuclear envelope. ...This stress treatment induced a disruption of the mitotic spindle, a reappearance and an extension of the …
Its two main characteristics are a very polarized spindle and a strong compartmentalization, ensured by large remnants of the …
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