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ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Ando Y, Elkayam E, McPherson RL, Dasovich M, Cheng SJ, Voorneveld J, Filippov DV, Ong SE, Joshua-Tor L, Leung AKL. Ando Y, et al. Mol Cell. 2019 Feb 21;73(4):845-856.e5. doi: 10.1016/j.molcel.2018.12.022. Epub 2019 Jan 31. Mol Cell. 2019. PMID: 30712989 Free PMC article.
ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. ...However, progress in this area is hindered by a lack of tools to investigate this protein modification. Here, we describe a new method named ELTA (enzymatic …
ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. ...However, progress in this ar …
Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis.
Badiee M, Boutonnet A, Phan D, Leung AKL. Badiee M, et al. Methods Mol Biol. 2023;2609:3-21. doi: 10.1007/978-1-0716-2891-1_1. Methods Mol Biol. 2023. PMID: 36515826 Free PMC article.
Poly(ADP-ribose) (PAR) is a homopolymer made of two or more adenosine diphosphate ribose (ADP-ribose) units. ...Briefly, we use the bioconjugation technique ELTA (enzymatic labeling of terminal ADP …
Poly(ADP-ribose) (PAR) is a homopolymer made of two or more adenosine diphosphate ribose (ADP-r …
Mitochondrial PARP1 regulates NAD(+)-dependent poly ADP-ribosylation of mitochondrial nucleoids.
Lee JH, Hussain M, Kim EW, Cheng SJ, Leung AKL, Fakouri NB, Croteau DL, Bohr VA. Lee JH, et al. Exp Mol Med. 2022 Dec;54(12):2135-2147. doi: 10.1038/s12276-022-00894-x. Epub 2022 Dec 6. Exp Mol Med. 2022. PMID: 36473936 Free PMC article.
However, very little is known about mitochondrial PARP (mtPARP) and poly ADP-ribosylation (PARylation). The existence of mtPARP is controversial, and the biological roles of mtPARP-induced mitochondrial PARylation are unclear. ...Mitochondrial PARylation was also evaluated …
However, very little is known about mitochondrial PARP (mtPARP) and poly ADP-ribosylation (PARylation). The existence of mtPARP is co …
dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms.
Uribe IR, Zahn E, Searfoss R, Kim HB, Dasovich M, Voorneveld J, Hunt SR, Onuoha UC, Valadez C, Filippov DV, Na CH, Garcia BA, Orsburn BC, Leung AKL. Uribe IR, et al. J Proteome Res. 2025 Apr 4;24(4):1791-1803. doi: 10.1021/acs.jproteome.4c00890. Epub 2025 Mar 13. J Proteome Res. 2025. PMID: 40079415 Free PMC article.
ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. ...Our method uses the coronaviral enzyme NS2 to reverse our previous labeling approach, …
ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MA …