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ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Ando Y, Elkayam E, McPherson RL, Dasovich M, Cheng SJ, Voorneveld J, Filippov DV, Ong SE, Joshua-Tor L, Leung AKL. Ando Y, et al. Mol Cell. 2019 Feb 21;73(4):845-856.e5. doi: 10.1016/j.molcel.2018.12.022. Epub 2019 Jan 31. Mol Cell. 2019. PMID: 30712989 Free PMC article.
ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. ...However, progress in this area is hindered by a lack of tools to investigate this protein modification. Here, we describe a new method named ELTA (enzymatic …
ADP-ribosylation refers to the addition of one or more ADP-ribose groups onto proteins. ...However, progress in this ar …
Mitochondrial PARP1 regulates NAD+-dependent poly ADP-ribosylation of mitochondrial nucleoids.
Lee JH, Hussain M, Kim EW, Cheng SJ, Leung AKL, Fakouri NB, Croteau DL, Bohr VA. Lee JH, et al. Exp Mol Med. 2022 Dec;54(12):2135-2147. doi: 10.1038/s12276-022-00894-x. Epub 2022 Dec 6. Exp Mol Med. 2022. PMID: 36473936 Free PMC article.
However, very little is known about mitochondrial PARP (mtPARP) and poly ADP-ribosylation (PARylation). The existence of mtPARP is controversial, and the biological roles of mtPARP-induced mitochondrial PARylation are unclear. ...The addition of the PARP1 substrate NAD(+) …
However, very little is known about mitochondrial PARP (mtPARP) and poly ADP-ribosylation (PARylation). The existence of mtPARP is co …
Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis.
Badiee M, Boutonnet A, Phan D, Leung AKL. Badiee M, et al. Methods Mol Biol. 2023;2609:3-21. doi: 10.1007/978-1-0716-2891-1_1. Methods Mol Biol. 2023. PMID: 36515826
Poly(ADP-ribose) (PAR) is a homopolymer made of two or more adenosine diphosphate ribose (ADP-ribose) units. ...Here, we describe several fluorescence-based methods that quantify PAR length distributions. Briefly, we use the biocon …
Poly(ADP-ribose) (PAR) is a homopolymer made of two or more adenosine diphosphate ribose (ADP-r …