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A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies.
Ng ES, Davis R, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: elefanty ag. Nat Protoc. 2008;3(5):768-76. doi: 10.1038/nprot.2008.42. Nat Protoc. 2008. PMID: 18451785
Hematopoietic-specific genes are not induced during in vitro differentiation of scl-null embryonic stem cells.
Elefanty AG, Robb L, Birner R, Begley CG. Elefanty AG, et al. Blood. 1997 Aug 15;90(4):1435-47. Blood. 1997. PMID: 9269761
Embryonic stem cells in the study of hematopoiesis.
Lazner F, Kola I, Stadler E, Elefanty AG. Lazner F, et al. Among authors: elefanty ag. Methods Mol Biol. 2001;158:287-300. doi: 10.1385/1-59259-220-1:287. Methods Mol Biol. 2001. PMID: 11236663 Review. No abstract available.
Retinoic acid induces Pdx1-positive endoderm in differentiating mouse embryonic stem cells.
Micallef SJ, Janes ME, Knezevic K, Davis RP, Elefanty AG, Stanley EG. Micallef SJ, et al. Among authors: elefanty ag. Diabetes. 2005 Feb;54(2):301-5. doi: 10.2337/diabetes.54.2.301. Epub 2004 Dec 7. Diabetes. 2005. PMID: 15585742
The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.
Ng ES, Azzola L, Sourris K, Robb L, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: elefanty ag. Development. 2005 Mar;132(5):873-84. doi: 10.1242/dev.01657. Epub 2005 Jan 26. Development. 2005. PMID: 15673572
The hESC line Envy expresses high levels of GFP in all differentiated progeny.
Costa M, Dottori M, Ng E, Hawes SM, Sourris K, Jamshidi P, Pera MF, Elefanty AG, Stanley EG. Costa M, et al. Among authors: elefanty ag. Nat Methods. 2005 Apr;2(4):259-60. doi: 10.1038/nmeth748. Epub 2005 Mar 23. Nat Methods. 2005. PMID: 15782217
Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation.
Ng ES, Davis RP, Azzola L, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: elefanty ag. Blood. 2005 Sep 1;106(5):1601-3. doi: 10.1182/blood-2005-03-0987. Epub 2005 May 24. Blood. 2005. PMID: 15914555
Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells.
Mossman AK, Sourris K, Ng E, Stanley EG, Elefanty AG. Mossman AK, et al. Among authors: elefanty ag. Stem Cells Dev. 2005 Dec;14(6):656-63. doi: 10.1089/scd.2005.14.656. Stem Cells Dev. 2005. PMID: 16433620
Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.
Hirst CE, Ng ES, Azzola L, Voss AK, Thomas T, Stanley EG, Elefanty AG. Hirst CE, et al. Among authors: elefanty ag. Dev Biol. 2006 May 1;293(1):90-103. doi: 10.1016/j.ydbio.2006.01.023. Epub 2006 Mar 20. Dev Biol. 2006. PMID: 16546155
A method for genetic modification of human embryonic stem cells using electroporation.
Costa M, Dottori M, Sourris K, Jamshidi P, Hatzistavrou T, Davis R, Azzola L, Jackson S, Lim SM, Pera M, Elefanty AG, Stanley EG. Costa M, et al. Among authors: elefanty ag. Nat Protoc. 2007;2(4):792-6. doi: 10.1038/nprot.2007.105. Nat Protoc. 2007. PMID: 17446878
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