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Enrichment of open reading frames presented on bacteriophage M13 using hyperphage.
Hust M, Meysing M, Schirrmann T, Selke M, Meens J, Gerlach GF, Dübel S. Hust M, et al. Biotechniques. 2006 Sep;41(3):335-42. doi: 10.2144/000112225. Biotechniques. 2006. PMID: 16989094 Free article.
The enrichment of open reading frames (ORFs) from large gene libraries and the presentation of the corresponding polypeptides on filamentous phage M13 (phage display) is frequently used to identify binding partners of unknown ORFs. ...Her …
The enrichment of open reading frames (ORFs) from large gene libraries and the presentation of the corres …
Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display.
Kügler J, Nieswandt S, Gerlach GF, Meens J, Schirrmann T, Hust M. Kügler J, et al. Appl Microbiol Biotechnol. 2008 Sep;80(3):447-58. doi: 10.1007/s00253-008-1576-1. Epub 2008 Jul 18. Appl Microbiol Biotechnol. 2008. PMID: 18636254
The fragmented genome of M. hyopneumoniae was cloned into a phage display vector, and the genomic library was packaged using the helperphage Hyperphage to enrich open reading frames (ORFs). Afterwards, the phage display library was screened by p …
The fragmented genome of M. hyopneumoniae was cloned into a phage display vector, and the genomic library was packaged using the helperphage …
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