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Multicenter benchmarking of short and long read wet lab protocols for clinical viral metagenomics.
Lopez-Labrador FX, Huber M, Sidorov IA, Brown JR, Cuypers L, Laenen L, Vanmechelen B, Maes P, Fischer N, Pichler I, Storey N, Atkinson L, Schmutz S, Kufner V, van Boheemen S, Mulders CE, Grundhoff A, Blümke P, Robitaille A, Cinek O, Hubáčková K, Mourik K, Boers SA, Stauber L, Salmona M, Cappy P, Ramette A, Franze' A, LeGoff J, Claas ECJ, Rodriguez C, de Vries JJC; European Society of Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS). Lopez-Labrador FX, et al. J Clin Virol. 2024 Aug;173:105695. doi: 10.1016/j.jcv.2024.105695. Epub 2024 May 25. J Clin Virol. 2024. PMID: 38823290 Free article.
In this study, we present a benchmark of metagenomics protocols used in clinical diagnostic laboratories initiated by the European Society for Clinical Virology (ESCV) Network on NGS (ENNGS). A mock viral reference panel was …
In this study, we present a benchmark of metagenomics protocols used in clinical diagnostic laboratories initiated by the European …
Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting.
de Vries JJC, Brown JR, Couto N, Beer M, Le Mercier P, Sidorov I, Papa A, Fischer N, Oude Munnink BB, Rodriquez C, Zaheri M, Sayiner A, Hönemann M, Cataluna AP, Carbo EC, Bachofen C, Kubacki J, Schmitz D, Tsioka K, Matamoros S, Höper D, Hernandez M, Puchhammer-Stöckl E, Lebrand A, Huber M, Simmonds P, Claas ECJ, López-Labrador FX; ESCV Network on Next-Generation Sequencing. de Vries JJC, et al. J Clin Virol. 2021 May;138:104812. doi: 10.1016/j.jcv.2021.104812. Epub 2021 Mar 26. J Clin Virol. 2021. PMID: 33819811 Free article.
Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into cl …
Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequenc …
Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure.
López-Labrador FX, Brown JR, Fischer N, Harvala H, Van Boheemen S, Cinek O, Sayiner A, Madsen TV, Auvinen E, Kufner V, Huber M, Rodriguez C, Jonges M, Hönemann M, Susi P, Sousa H, Klapper PE, Pérez-Cataluňa A, Hernandez M, Molenkamp R, der Hoek LV, Schuurman R, Couto N, Leuzinger K, Simmonds P, Beer M, Höper D, Kamminga S, Feltkamp MCW, Rodríguez-Díaz J, Keyaerts E, Nielsen XC, Puchhammer-Stöckl E, Kroes ACM, Buesa J, Breuer J, Claas ECJ, de Vries JJC; ESCV Network on Next-Generation Sequencing. López-Labrador FX, et al. J Clin Virol. 2021 Jan;134:104691. doi: 10.1016/j.jcv.2020.104691. Epub 2020 Nov 18. J Clin Virol. 2021. PMID: 33278791 Free article.
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into …
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determin …
Benchmark of thirteen bioinformatic pipelines for metagenomic virus diagnostics using datasets from clinical samples.
de Vries JJC, Brown JR, Fischer N, Sidorov IA, Morfopoulou S, Huang J, Munnink BBO, Sayiner A, Bulgurcu A, Rodriguez C, Gricourt G, Keyaerts E, Beller L, Bachofen C, Kubacki J, Samuel C, Florian L, Dennis S, Beer M, Hoeper D, Huber M, Kufner V, Zaheri M, Lebrand A, Papa A, van Boheemen S, Kroes ACM, Breuer J, Lopez-Labrador FX, Claas ECJ. de Vries JJC, et al. J Clin Virol. 2021 Aug;141:104908. doi: 10.1016/j.jcv.2021.104908. Epub 2021 Jul 8. J Clin Virol. 2021. PMID: 34273858 Free PMC article.
INTRODUCTION: Metagenomic sequencing is increasingly being used in clinical settings for difficult to diagnose cases. The performance of viral metagenomic protocols relies to a large extent on the bioinformatic analysis. In this study, the European Society …
INTRODUCTION: Metagenomic sequencing is increasingly being used in clinical settings for difficult to diagnose cases. The perf …