Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell-transcriptomics.
J Cell Physiol. 2020 Jun;235(6):5241-5255. doi: 10.1002/jcp.29411. Epub 2019 Dec 16.
J Cell Physiol. 2020.
PMID: 31840817
Free PMC article.
Here, we describe a simple, two-step differentiation strategy using a cocktail of four factors (LDN, AGN, FGF, and CHIR) for efficient derivation of notochordal cells from human embryonic stem cells (hESCs). We employed a CRISPR/Cas9 based genome-editi …
Here, we describe a simple, two-step differentiation strategy using a cocktail of four factors (LDN, AGN, FGF, and CHIR) for efficient deriv …