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Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell-transcriptomics.
Diaz-Hernandez ME, Khan NM, Trochez CM, Yoon T, Maye P, Presciutti SM, Gibson G, Drissi H. Diaz-Hernandez ME, et al. J Cell Physiol. 2020 Jun;235(6):5241-5255. doi: 10.1002/jcp.29411. Epub 2019 Dec 16. J Cell Physiol. 2020. PMID: 31840817 Free PMC article.
Here, we describe a simple, two-step differentiation strategy using a cocktail of four factors (LDN, AGN, FGF, and CHIR) for efficient derivation of notochordal cells from human embryonic stem cells (hESCs). We employed a CRISPR/Cas9 based genome-editi …
Here, we describe a simple, two-step differentiation strategy using a cocktail of four factors (LDN, AGN, FGF, and CHIR) for efficient deriv …
Differences between human and mouse embryonic stem cells.
Ginis I, Luo Y, Miura T, Thies S, Brandenberger R, Gerecht-Nir S, Amit M, Hoke A, Carpenter MK, Itskovitz-Eldor J, Rao MS. Ginis I, et al. Dev Biol. 2004 May 15;269(2):360-80. doi: 10.1016/j.ydbio.2003.12.034. Dev Biol. 2004. PMID: 15110706 Free article.
These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin …
These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the exp …