Decreased ecto-NTPDase1/CD39 activity leads to desensitization of P2 purinoceptors regulating tonus of corpora cavernosa in impotent men with endothelial dysfunction

M Faria; T Magalhães-Cardoso; J-M Lafuente-de-Carvalho; P Correia-de Sá

doi:10.1080/15257770802145744

Decreased ecto-NTPDase1/CD39 activity leads to desensitization of P2 purinoceptors regulating tonus of corpora cavernosa in impotent men with endothelial dysfunction

Nucleosides Nucleotides Nucleic Acids. 2008 Jun;27(6):761-8. doi: 10.1080/15257770802145744.

Authors

M Faria¹, T Magalhães-Cardoso, J-M Lafuente-de-Carvalho, P Correia-de Sá

Affiliation

¹ Laboratório de Farmacologia e Neurobiologia/UMIB, Instituto de Ciências Biomédicas Abel Salazar-Universidade do Porto (ICBAS-UP), Porto, Poturgal.

PMID: 18600538
DOI: 10.1080/15257770802145744

Abstract

Vascular responses to adenine nucleotides in human corpora cavernosa from men with vasculogenic erectile dysfunction were investigated. We also evaluated the catabolism of extracellular adenine nucleotides to probe its relevance to vascular hemodynamics in impotent men. Human corpora cavernosa have high NTPDase1/CD39 activity, converting ATP directly into AMP, without significant ADP formation. Extracellular ATP hydrolysis is slower in impotent patients. Adenine nucleotides have dual roles on phenylephrine-contracted strips of corpora cavernosa operated by P2X-contractant and P2Y-relaxant receptors. Prolonged exposure to endogenous ATP related to decreased NTPDase1/CD39 activity leads to P2-purinoceptor desensitization in impotent men. Shutting down ATP signaling in vasculogenic impotent men may represent a defense mechanism for preventing purinergic overstimulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine A2 Receptor Agonists
Adenosine Triphosphate / analogs & derivatives
Adenosine Triphosphate / metabolism
Adenosine Triphosphate / pharmacology
Adolescent
Adult
Antigens, CD / metabolism*
Apyrase / metabolism*
Case-Control Studies
Dose-Response Relationship, Drug
Endothelium / blood supply
Endothelium / enzymology
Endothelium / metabolism
Endothelium / physiopathology*
Humans
Impotence, Vasculogenic / enzymology
Impotence, Vasculogenic / metabolism
Impotence, Vasculogenic / physiopathology*
Male
Middle Aged
Muscle Contraction / drug effects
Muscle Tonus / drug effects
Muscle Tonus / physiology*
Penis / blood supply*
Penis / drug effects
Penis / metabolism
Penis / physiopathology*
Receptor, Adenosine A2B / metabolism
Receptors, Purinergic P2 / metabolism*
Signal Transduction / drug effects

Substances

Adenosine A2 Receptor Agonists
Antigens, CD
Receptor, Adenosine A2B
Receptors, Purinergic P2
adenosine 5'-O-(3-thiotriphosphate)
Adenosine Triphosphate
Apyrase
CD39 antigen
alpha,beta-methyleneadenosine 5'-triphosphate