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Hexahistidine (His6)-tag dependent protein dimerization: a cautionary tale.
Wu J, Filutowicz M. Wu J, et al. Among authors: filutowicz m. Acta Biochim Pol. 1999;46(3):591-9. Acta Biochim Pol. 1999. PMID: 10698267
Binding modes of the initiator and inhibitor forms of the replication protein pi to the gamma ori iteron of plasmid R6K.
Kunnimalaiyaan S, Krüger R, Ross W, Rakowski SA, Filutowicz M. Kunnimalaiyaan S, et al. Among authors: filutowicz m. J Biol Chem. 2004 Sep 24;279(39):41058-66. doi: 10.1074/jbc.M403151200. Epub 2004 Jul 9. J Biol Chem. 2004. PMID: 15247259
Plasmid R6K replication control.
Rakowski SA, Filutowicz M. Rakowski SA, et al. Among authors: filutowicz m. Plasmid. 2013 May;69(3):231-42. doi: 10.1016/j.plasmid.2013.02.003. Epub 2013 Mar 5. Plasmid. 2013. PMID: 23474464 Free PMC article. Review.
Bacterial expression system with tightly regulated gene expression and plasmid copy number.
Bowers LM, Lapoint K, Anthony L, Pluciennik A, Filutowicz M. Bowers LM, et al. Among authors: filutowicz m. Gene. 2004 Sep 29;340(1):11-8. doi: 10.1016/j.gene.2004.06.012. Gene. 2004. PMID: 15556290
Cooperative binding mode of the inhibitors of R6K replication, pi dimers.
Bowers LM, Filutowicz M. Bowers LM, et al. Among authors: filutowicz m. J Mol Biol. 2008 Mar 28;377(3):609-15. doi: 10.1016/j.jmb.2008.01.039. Epub 2008 Jan 26. J Mol Biol. 2008. PMID: 18295232 Free PMC article.
Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.
Kunnimalaiyaan S, Rakowski SA, Filutowicz M. Kunnimalaiyaan S, et al. Among authors: filutowicz m. J Bacteriol. 2007 Jul;189(13):4953-6. doi: 10.1128/JB.00109-07. Epub 2007 Apr 20. J Bacteriol. 2007. PMID: 17449630 Free PMC article.
Mechanism of origin activation by monomers of R6K-encoded pi protein.
Bowers LM, Krüger R, Filutowicz M. Bowers LM, et al. Among authors: filutowicz m. J Mol Biol. 2007 May 11;368(4):928-38. doi: 10.1016/j.jmb.2007.02.074. Epub 2007 Mar 2. J Mol Biol. 2007. PMID: 17383678 Free PMC article.
Small deletion variants of the replication protein, pi, and their potential for over-replication-based antimicrobial activity.
Peng Y, Rakowski SA, Filutowicz M. Peng Y, et al. Among authors: filutowicz m. FEMS Microbiol Lett. 2006 Aug;261(2):245-52. doi: 10.1111/j.1574-6968.2006.00364.x. FEMS Microbiol Lett. 2006. PMID: 16907728 Free article.
Role of pi dimers in coupling ("handcuffing") of plasmid R6K's gamma ori iterons.
Kunnimalaiyaan S, Inman RB, Rakowski SA, Filutowicz M. Kunnimalaiyaan S, et al. Among authors: filutowicz m. J Bacteriol. 2005 Jun;187(11):3779-85. doi: 10.1128/JB.187.11.3779-3785.2005. J Bacteriol. 2005. PMID: 15901701 Free PMC article.
Characterization of His-tagged, R6K-encoded pi protein variants.
Krüger R, Filutowicz M. Krüger R, et al. Among authors: filutowicz m. Plasmid. 2003 Jul;50(1):80-5. doi: 10.1016/s0147-619x(03)00043-x. Plasmid. 2003. PMID: 12826061
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