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dsRNA-mediated gene silencing in cultured Drosophila cells: a tissue culture model for the analysis of RNA interference.
Caplen NJ, Fleenor J, Fire A, Morgan RA. Caplen NJ, et al. Gene. 2000 Jul 11;252(1-2):95-105. doi: 10.1016/s0378-1119(00)00224-9. Gene. 2000. PMID: 10903441
RNA interference (RNAi) is a form of post-transcriptional gene silencing that has been described in a number of plant, nematode, protozoan, and invertebrate species. RNAi is characterized by a number of features: induction by double stranded RNA (dsRNA), a
RNA interference (RNAi) is a form of post-transcriptional gene silencing that has been described in a number of plant, nematod …
A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans.
Fire A, Harrison SW, Dixon D. Fire A, et al. Gene. 1990 Sep 14;93(2):189-98. doi: 10.1016/0378-1119(90)90224-f. Gene. 1990. PMID: 2121610
We describe a series of plasmid vectors which contain modular features particularly useful for studying gene expression in eukaryotic systems. The vectors contain the Escherichia coli beta-galactosidase (beta Gal)-encoding region (the lacZ gene) flanked by unique polylinke …
We describe a series of plasmid vectors which contain modular features particularly useful for studying gene expression in eukaryotic …
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Fire A, et al. Nature. 1998 Feb 19;391(6669):806-11. doi: 10.1038/35888. Nature. 1998. PMID: 9486653
After injection into adult animals, purified single strands had at most a modest effect, whereas double-stranded mixtures caused potent and specific interference. ...Only a few molecules of injected double-stranded RNA were required per affected cell, arguing agains …
After injection into adult animals, purified single strands had at most a modest effect, whereas double-stranded mixtures caused pote …
Chromatin silencing and the maintenance of a functional germline in Caenorhabditis elegans.
Kelly WG, Fire A. Kelly WG, et al. Development. 1998 Jul;125(13):2451-6. Development. 1998. PMID: 9609828 Free PMC article.
The germline of the nematode Caenorhabditis elegans exhibits a remarkable ability to specifically silence transgenic DNA. We have shown that this silencing mechanism is disrupted in animals mutant for the maternal effect sterile genes mes-2, mes-3, mes-4 and mes-6. ...Thes …
The germline of the nematode Caenorhabditis elegans exhibits a remarkable ability to specifically silence transgenic DNA. We have sho …
RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans.
Montgomery MK, Xu S, Fire A. Montgomery MK, et al. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15502-7. doi: 10.1073/pnas.95.26.15502. Proc Natl Acad Sci U S A. 1998. PMID: 9860998 Free PMC article.
These results indicate that the endogenous mRNA is the target for interference and suggest a mechanism that degrades the targeted RNA before translation can occur. ...We suggest a model of how dsRNA might function in a catalytic mechanism to target homologous …
These results indicate that the endogenous mRNA is the target for interference and suggest a mechanism that degrades the targeted RNA …
Evolutionary conservation of MyoD function and differential utilization of E proteins.
Zhang JM, Chen L, Krause M, Fire A, Paterson BM. Zhang JM, et al. Dev Biol. 1999 Apr 15;208(2):465-72. doi: 10.1006/dbio.1999.9218. Dev Biol. 1999. PMID: 10191059
A second assessment of conserved function comes from the ability of these factors to rescue a C. elegans hlh-1 (CeMyoD) null mutation. We found that both Drosophila and chicken MyoD-related factors were able to rescue a C. elegans CeMyoD loss-of-function muta
A second assessment of conserved function comes from the ability of these factors to rescue a C. elegans hlh-1 (CeMyoD) null m
RNA-triggered gene silencing.
Fire A. Fire A. Trends Genet. 1999 Sep;15(9):358-63. doi: 10.1016/s0168-9525(99)01818-1. Trends Genet. 1999. PMID: 10461204 Review.
Double-stranded RNA (dsRNA) has recently been shown to trigger sequence-specific gene silencing in a wide variety of organisms, including nematodes, plants, trypanosomes, fruit flies and planaria; meanwhile an as yet uncharacterized RNA trigger has been shown to induce DNA …
Double-stranded RNA (dsRNA) has recently been shown to trigger sequence-specific gene silencing in a wide variety of organisms, inclu …
The rde-1 gene, RNA interference, and transposon silencing in C. elegans.
Tabara H, Sarkissian M, Kelly WG, Fleenor J, Grishok A, Timmons L, Fire A, Mello CC. Tabara H, et al. Cell. 1999 Oct 15;99(2):123-32. doi: 10.1016/s0092-8674(00)81644-x. Cell. 1999. PMID: 10535731
We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates. Interestingly, several, but not all, RNAi-deficient strains exhibit mobilization of the endogenous transposons. ...
We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates. Interesting …
Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference.
Parrish S, Fleenor J, Xu S, Mello C, Fire A. Parrish S, et al. Mol Cell. 2000 Nov;6(5):1077-87. doi: 10.1016/s1097-2765(00)00106-4. Mol Cell. 2000. PMID: 11106747
We show that RNAi requires duplex formation between the two trigger strands, that the duplex must include a region of identity between trigger and target RNAs, and that duplexes as short as 26 bp can trigger RNAi. Consistent with in vitro observations, a fraction of …
We show that RNAi requires duplex formation between the two trigger strands, that the duplex must include a region of identity betwee …
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