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Identification of Effectors: Precipitation of Supernatant Material.
Flaugnatti N, Journet L. Flaugnatti N, et al. Methods Mol Biol. 2017;1615:459-464. doi: 10.1007/978-1-4939-7033-9_31. Methods Mol Biol. 2017. PMID: 28667629
A phospholipase A1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery.
Flaugnatti N, Le TT, Canaan S, Aschtgen MS, Nguyen VS, Blangy S, Kellenberger C, Roussel A, Cambillau C, Cascales E, Journet L. Flaugnatti N, et al. Mol Microbiol. 2016 Mar;99(6):1099-118. doi: 10.1111/mmi.13292. Epub 2016 Feb 10. Mol Microbiol. 2016. PMID: 26714038 Free article.
Priming and polymerization of a bacterial contractile tail structure.
Zoued A, Durand E, Brunet YR, Spinelli S, Douzi B, Guzzo M, Flaugnatti N, Legrand P, Journet L, Fronzes R, Mignot T, Cambillau C, Cascales E. Zoued A, et al. Among authors: flaugnatti n. Nature. 2016 Mar 3;531(7592):59-63. doi: 10.1038/nature17182. Epub 2016 Feb 24. Nature. 2016. PMID: 26909579
Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut.
Sana TG, Flaugnatti N, Lugo KA, Lam LH, Jacobson A, Baylot V, Durand E, Journet L, Cascales E, Monack DM. Sana TG, et al. Among authors: flaugnatti n. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51. doi: 10.1073/pnas.1608858113. Epub 2016 Aug 8. Proc Natl Acad Sci U S A. 2016. PMID: 27503894 Free PMC article.
Structure and Activity of the Type VI Secretion System.
Cherrak Y, Flaugnatti N, Durand E, Journet L, Cascales E. Cherrak Y, et al. Among authors: flaugnatti n. Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.PSIB-0031-2019. Microbiol Spectr. 2019. PMID: 31298206 Review.
Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike.
Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot-Rooke J, Cascales E, Fronzes R, Journet L. Flaugnatti N, et al. EMBO J. 2020 Jun 2;39(11):e104129. doi: 10.15252/embj.2019104129. Epub 2020 Apr 30. EMBO J. 2020. PMID: 32350888