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Pyrophosphate and tripolyphosphate affect firefly luciferase luminescence because they act as substrates and not as allosteric effectors.
Fontes R, Fernandes D, Peralta F, Fraga H, Maio I, Esteves da Silva JC. Fontes R, et al. FEBS J. 2008 Apr;275(7):1500-9. doi: 10.1111/j.1742-4658.2008.06309.x. Epub 2008 Feb 14. FEBS J. 2008. PMID: 18279384
Synthesis of dehydroluciferin by firefly luciferase: effect of dehydroluciferin, coenzyme A and nucleoside triphosphates on the luminescent reaction.
Fontes R, Dukhovich A, Sillero A, Sillero MA. Fontes R, et al. Biochem Biophys Res Commun. 1997 Aug 18;237(2):445-50. doi: 10.1006/bbrc.1997.7161. Biochem Biophys Res Commun. 1997. PMID: 9268731
Acyl-CoA synthetase catalyzes the synthesis of diadenosine hexaphosphate (Ap6A).
Fontes R, Günther Sillero MA, Sillero A. Fontes R, et al. Biochimie. 1999 Mar;81(3):229-33. doi: 10.1016/s0300-9084(99)80056-x. Biochimie. 1999. PMID: 10385004
Enzymatic synthesis of mono and dinucleoside polyphosphates.
Fraga H, Fontes R. Fraga H, et al. Biochim Biophys Acta. 2011 Dec;1810(12):1195-204. doi: 10.1016/j.bbagen.2011.09.010. Epub 2011 Sep 25. Biochim Biophys Acta. 2011. PMID: 21978831 Review.
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