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Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications.
Branchini BR, Ablamsky DM, Murtiashaw MH, Uzasci L, Fraga H, Southworth TL. Branchini BR, et al. Anal Biochem. 2007 Feb 15;361(2):253-62. doi: 10.1016/j.ab.2006.10.043. Epub 2006 Nov 21. Anal Biochem. 2007. PMID: 17181991
Firefly luminescence: a historical perspective and recent developments.
Fraga H. Fraga H. Photochem Photobiol Sci. 2008 Feb;7(2):146-58. doi: 10.1039/b719181b. Epub 2008 Jan 23. Photochem Photobiol Sci. 2008. PMID: 18264582 Review.
Pyrophosphate and tripolyphosphate affect firefly luciferase luminescence because they act as substrates and not as allosteric effectors.
Fontes R, Fernandes D, Peralta F, Fraga H, Maio I, Esteves da Silva JC. Fontes R, et al. FEBS J. 2008 Apr;275(7):1500-9. doi: 10.1111/j.1742-4658.2008.06309.x. Epub 2008 Feb 14. FEBS J. 2008. PMID: 18279384
FH8--a small EF-hand protein from Fasciola hepatica.
Fraga H, Faria TQ, Pinto F, Almeida A, Brito RM, Damas AM. Fraga H, et al. FEBS J. 2010 Dec;277(24):5072-85. doi: 10.1111/j.1742-4658.2010.07912.x. Epub 2010 Nov 16. FEBS J. 2010. PMID: 21078120
Enzymatic synthesis of mono and dinucleoside polyphosphates.
Fraga H, Fontes R. Fraga H, et al. Biochim Biophys Acta. 2011 Dec;1810(12):1195-204. doi: 10.1016/j.bbagen.2011.09.010. Epub 2011 Sep 25. Biochim Biophys Acta. 2011. PMID: 21978831 Review.
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