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The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA.
Garneau JE, Dupuis MÈ, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S. Garneau JE, et al. Nature. 2010 Nov 4;468(7320):67-71. doi: 10.1038/nature09523. Nature. 2010. PMID: 21048762
Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system.
Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. Sinkunas T, et al. EMBO J. 2011 Apr 6;30(7):1335-42. doi: 10.1038/emboj.2011.41. Epub 2011 Feb 22. EMBO J. 2011. PMID: 21343909 Free PMC article.
Cas3 contains N-terminal HD phosphohydrolase and C-terminal Superfamily 2 (SF2) helicase domains. Here, we provide the first report of the cloning, expression, purification and in vitro functional analysis of the Cas3 protein of the Streptococcus thermophilus CRISPR4 (Ecol …
Cas3 contains N-terminal HD phosphohydrolase and C-terminal Superfamily 2 (SF2) helicase domains. Here, we provide the first report o …
The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli.
Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. Sapranauskas R, et al. Nucleic Acids Res. 2011 Nov;39(21):9275-82. doi: 10.1093/nar/gkr606. Epub 2011 Aug 3. Nucleic Acids Res. 2011. PMID: 21813460 Free PMC article.
Genomic organization and molecular analysis of virulent bacteriophage 2972 infecting an exopolysaccharide-producing Streptococcus thermophilus strain.
Lévesque C, Duplessis M, Labonté J, Labrie S, Fremaux C, Tremblay D, Moineau S. Lévesque C, et al. Appl Environ Microbiol. 2005 Jul;71(7):4057-68. doi: 10.1128/AEM.71.7.4057-4068.2005. Appl Environ Microbiol. 2005. PMID: 16000821 Free PMC article.
The genome of phage 2972 has 34,704 bp with an overall G+C content of 40.15%, making it the shortest S. thermophilus phage genome analyzed so far. ...
The genome of phage 2972 has 34,704 bp with an overall G+C content of 40.15%, making it the shortest S. thermophilus phage genome ana …
Diversity, activity, and evolution of CRISPR loci in Streptococcus thermophilus.
Horvath P, Romero DA, Coûté-Monvoisin AC, Richards M, Deveau H, Moineau S, Boyaval P, Fremaux C, Barrangou R. Horvath P, et al. J Bacteriol. 2008 Feb;190(4):1401-12. doi: 10.1128/JB.01415-07. Epub 2007 Dec 7. J Bacteriol. 2008. PMID: 18065539 Free PMC article.
Phage mutations in response to CRISPR diversification in a bacterial population.
Sun CL, Barrangou R, Thomas BC, Horvath P, Fremaux C, Banfield JF. Sun CL, et al. Among authors: fremaux c. Environ Microbiol. 2013 Feb;15(2):463-70. doi: 10.1111/j.1462-2920.2012.02879.x. Epub 2012 Oct 12. Environ Microbiol. 2013. PMID: 23057534
Phage-induced expression of CRISPR-associated proteins is revealed by shotgun proteomics in Streptococcus thermophilus.
Young JC, Dill BD, Pan C, Hettich RL, Banfield JF, Shah M, Fremaux C, Horvath P, Barrangou R, Verberkmoes NC. Young JC, et al. Among authors: fremaux c. PLoS One. 2012;7(5):e38077. doi: 10.1371/journal.pone.0038077. Epub 2012 May 30. PLoS One. 2012. PMID: 22666452 Free PMC article.
CRISPR provides acquired resistance against viruses in prokaryotes.
Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero DA, Horvath P. Barrangou R, et al. Science. 2007 Mar 23;315(5819):1709-12. doi: 10.1126/science.1138140. Science. 2007. PMID: 17379808
Phage response to CRISPR-encoded resistance in Streptococcus thermophilus.
Deveau H, Barrangou R, Garneau JE, Labonté J, Fremaux C, Boyaval P, Romero DA, Horvath P, Moineau S. Deveau H, et al. J Bacteriol. 2008 Feb;190(4):1390-400. doi: 10.1128/JB.01412-07. Epub 2007 Dec 7. J Bacteriol. 2008. PMID: 18065545 Free PMC article.
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.
Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Hynes AP, et al. Among authors: fremaux c. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. Nat Commun. 2018. PMID: 30046034 Free PMC article.
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