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The mediator for stringent control, ppGpp, binds to the beta-subunit of Escherichia coli RNA polymerase.
Chatterji D, Fujita N, Ishihama A. Chatterji D, et al. Genes Cells. 1998 May;3(5):279-87. doi: 10.1046/j.1365-2443.1998.00190.x. Genes Cells. 1998. PMID: 9685179
CONCLUSION: It was observed that both the N-terminal and C-terminal domains of the beta-subunit were labelled with azido-ppGpp in the native enzyme. ...
CONCLUSION: It was observed that both the N-terminal and C-terminal domains of the beta-subunit were labelled with azido-ppGpp in the …
Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region.
Belyaeva TA, Bown JA, Fujita N, Ishihama A, Busby SJ. Belyaeva TA, et al. Nucleic Acids Res. 1996 Jun 15;24(12):2242-51. doi: 10.1093/nar/24.12.2243. Nucleic Acids Res. 1996. PMID: 8710492 Free PMC article.
Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at class II promoters.
West D, Williams R, Rhodius V, Bell A, Sharma N, Zou C, Fujita N, Ishihama A, Busby S. West D, et al. Mol Microbiol. 1993 Nov;10(4):789-97. doi: 10.1111/j.1365-2958.1993.tb00949.x. Mol Microbiol. 1993. PMID: 7934841
Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma 38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli.
Tanaka K, Takayanagi Y, Fujita N, Ishihama A, Takahashi H. Tanaka K, et al. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3511-5. doi: 10.1073/pnas.90.8.3511. Proc Natl Acad Sci U S A. 1993. PMID: 8475100 Free PMC article.
Physical mapping of a collection of Mael-generating amber mutations in the beta gene of Escherichia coli RNA polymerase and the functional effect of internal deletions constructed through their manipulation.
Buyukuslu N, Trigwell SM, Lim PP, Fujita N, Ishihama A, Ralphs NT, Glass RE. Buyukuslu N, et al. Genes Funct. 1997 Apr;1(2):119-29. doi: 10.1046/j.1365-4624.1997.00013.x. Genes Funct. 1997. PMID: 9680313
The lack of amber fragments for mutations within the 5' approx. 265 codons suggests lability of the extreme N-terminal region; further potential destabilizing 'signals' may be present in the non-conserved 'spacer' regions. ...These polar amber sites define three different …
The lack of amber fragments for mutations within the 5' approx. 265 codons suggests lability of the extreme N-terminal region; furthe …
Mapping the sigma70 subunit contact sites on Escherichia coli RNA polymerase with a sigma70-conjugated chemical protease.
Owens JT, Miyake R, Murakami K, Chmura AJ, Fujita N, Ishihama A, Meares CF. Owens JT, et al. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6021-6. doi: 10.1073/pnas.95.11.6021. Proc Natl Acad Sci U S A. 1998. PMID: 9600910 Free PMC article.
Competition among seven Escherichia coli sigma subunits: relative binding affinities to the core RNA polymerase.
Maeda H, Fujita N, Ishihama A. Maeda H, et al. Nucleic Acids Res. 2000 Sep 15;28(18):3497-503. doi: 10.1093/nar/28.18.3497. Nucleic Acids Res. 2000. PMID: 10982868 Free PMC article.
Mixed reconstitution experiments in the presence of a fixed amount of core enzyme and increasing amounts of an equimolar mixture of all seven sigma subunits indicated that sigma(70) is strongest in terms of core enzyme binding, followed by sigma(N), sigma(F), sigma(E)/sigm …
Mixed reconstitution experiments in the presence of a fixed amount of core enzyme and increasing amounts of an equimolar mixture of all seve …
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