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Dissociation of embryonic kidney followed by re-aggregation as a method for chimeric analysis.
Davies JA, Unbekandt M, Ineson J, Lusis M, Little MH. Davies JA, et al. Methods Mol Biol. 2012;886:135-46. doi: 10.1007/978-1-61779-851-1_12. Methods Mol Biol. 2012. PMID: 22639257
This chapter presents three methods for re-constructing mouse foetal kidney tissue from simple suspensions of cells. These techniques are very useful for a number of purposes: (1) they allow the production of fine-grained chimaeras in which cell autonomy of mutations can b …
This chapter presents three methods for re-constructing mouse foetal kidney tissue from simple suspensions of cells. These techniques are ve …
An improved method of renal tissue engineering, by combining renal dissociation and reaggregation with a low-volume culture technique, results in development of engineered kidneys complete with loops of Henle.
Chang CH, Davies JA. Chang CH, et al. Nephron Exp Nephrol. 2012;121(3-4):e79-85. doi: 10.1159/000345514. Epub 2012 Dec 7. Nephron Exp Nephrol. 2012. PMID: 23235540
The previous state-of-the-art method for this results in the formation of a branched collecting duct tree, immature nephrons (S-shaped bodies) beside and connected to it, and supportive stroma. ...
The previous state-of-the-art method for this results in the formation of a branched collecting duct tree, immature nephrons (S-shape …
Dissociation of embryonic kidneys followed by reaggregation allows the formation of renal tissues.
Unbekandt M, Davies JA. Unbekandt M, et al. Kidney Int. 2010 Mar;77(5):407-16. doi: 10.1038/ki.2009.482. Epub 2009 Dec 16. Kidney Int. 2010. PMID: 20016472 Free article.
Chimeric renal cultures were formed using mixtures of unmarked normal host embryonic kidney cells and CellTracker-marked WT1 siRNA-carrying cells to test the hypothesis that WT1 is important to a cell's ability to contribute to nephron formation. We found a significant red …
Chimeric renal cultures were formed using mixtures of unmarked normal host embryonic kidney cells and CellTracker-marked WT1 siRNA-carrying …
A novel, low-volume method for organ culture of embryonic kidneys that allows development of cortico-medullary anatomical organization.
Sebinger DD, Unbekandt M, Ganeva VV, Ofenbauer A, Werner C, Davies JA. Sebinger DD, et al. PLoS One. 2010 May 10;5(5):e10550. doi: 10.1371/journal.pone.0010550. PLoS One. 2010. PMID: 20479933 Free PMC article.
It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they require relatively large volumes (1-3 mls) of culture medium, which can make high-throughput screens expensive, they require porous (fi …
It recapitulates many aspects of early development very well, but the established techniques have some disadvantages: in particular, they re …
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