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584 results

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Page 1
Preservation of exosomes at room temperature using lyophilization.
Charoenviriyakul C, Takahashi Y, Nishikawa M, Takakura Y. Charoenviriyakul C, et al. Int J Pharm. 2018 Dec 20;553(1-2):1-7. doi: 10.1016/j.ijpharm.2018.10.032. Epub 2018 Oct 11. Int J Pharm. 2018. PMID: 30316791
Lyophilization had little effect on the pharmacokinetics of Gaussia luciferase (gLuc)-labeled exosomes after an intravenous injection into mice. ...
Lyophilization had little effect on the pharmacokinetics of Gaussia luciferase (gLuc)-labeled exosomes after an intravenous injection …
Gaussia princeps luciferase.
Chopra A. Chopra A. 2008 Jan 31 [updated 2008 Feb 28]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. 2008 Jan 31 [updated 2008 Feb 28]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. PMID: 20641352 Free Books & Documents. Review.
Tannous et al. decided to explore the possibility of finding a superior luciferase that was more efficient and had a higher quantum yield compared with the FLuc and RLuc enzymes (12). Gaussia princeps, a marine copepod, naturally secretes a luciferase (GLuc) that was clone …
Tannous et al. decided to explore the possibility of finding a superior luciferase that was more efficient and had a higher quantum yield co …
Optimized signal peptides for the development of high expressing CHO cell lines.
Kober L, Zehe C, Bode J. Kober L, et al. Biotechnol Bioeng. 2013 Apr;110(4):1164-73. doi: 10.1002/bit.24776. Epub 2013 Jan 17. Biotechnol Bioeng. 2013. PMID: 23124363
A major aim of our study was therefore to improve the secretion efficiency of a recombinant biotherapeutic antibody by optimizing signal peptides. Reporter molecules such as gaussia and vargula luciferase or secreted alkaline phosphatase are frequently used to this end. .. …
A major aim of our study was therefore to improve the secretion efficiency of a recombinant biotherapeutic antibody by optimizing signal pep …
Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase.
Wang L, Cui Q, Zhao X, Li P, Wang Y, Rong L, Du R. Wang L, et al. Viruses. 2019 Jul 20;11(7):665. doi: 10.3390/v11070665. Viruses. 2019. PMID: 31330768 Free PMC article.
In this study, the segments of A/Wyoming/3/03 (NY, H3N2) virus encoding hemagglutinin and neuraminidase, respectively, were reassorted with the six internal genes of PR8 where the NS gene was fused with a Gaussia luciferase (Gluc) gene. Using reverse genetics, NY-r19-Gluc, …
In this study, the segments of A/Wyoming/3/03 (NY, H3N2) virus encoding hemagglutinin and neuraminidase, respectively, were reassorted with …
Bacterial expression and re-engineering of Gaussia princeps luciferase and its use as a reporter protein.
Wu N, Rathnayaka T, Kuroda Y. Wu N, et al. Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1392-9. doi: 10.1016/j.bbapap.2015.05.008. Epub 2015 May 27. Biochim Biophys Acta. 2015. PMID: 26025768 Review.
Some luciferases, such as those from fireflies and Renilla, have been thoroughly characterized and are being used in a wide range of applications in bio-imaging. Gaussia luciferase (GLuc) from the marine copepod Gaussia princeps is the smallest known luciferase, and …
Some luciferases, such as those from fireflies and Renilla, have been thoroughly characterized and are being used in a wide range of applica …
Reflecting on mutational and biophysical analysis of Gaussia princeps Luciferase from a structural perspective: a unique bioluminescent enzyme.
Wu N, Kobayashi N, Kuroda Y, Yamazaki T. Wu N, et al. Biophys Rev. 2022 Dec 14;14(6):1513-1520. doi: 10.1007/s12551-022-01025-6. eCollection 2022 Dec. Biophys Rev. 2022. PMID: 36659992 Free PMC article. Review.
Gaussia princeps luciferase (GLuc 18.2 kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizing coelenterazine (CTZ). ...
Gaussia princeps luciferase (GLuc 18.2 kDa; 168 residues) is a marine copepod luciferase that emits a bright blue light when oxidizin …
Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture.
Degeling MH, Bovenberg MS, Tannous M, Tannous BA. Degeling MH, et al. Methods Mol Biol. 2014;1098:47-55. doi: 10.1007/978-1-62703-718-1_4. Methods Mol Biol. 2014. PMID: 24166367
In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter....
In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia …
Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus.
Blower I, Tong C, Sun X, Murray E, Luckett J, Chan W, Williams P, Hill P. Blower I, et al. Sensors (Basel). 2020 Aug 1;20(15):4305. doi: 10.3390/s20154305. Sensors (Basel). 2020. PMID: 32752273 Free PMC article.
Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. ...Use of GLuc enabled quantitative assessment of agr activity. This demonstrates the utility of Gaussia luciferas …
Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial path …
584 results