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Elimination of redundant and stop codons during the chemical synthesis of degenerate oligonucleotides. Combinatorial testing on the chromophore region of the red fluorescent protein mKate.
Gaytán P, Roldán-Salgado A. Gaytán P, et al. ACS Synth Biol. 2013 Aug 16;2(8):453-62. doi: 10.1021/sb3001326. Epub 2013 Feb 13. ACS Synth Biol. 2013. PMID: 23654278
Structural Factors Enabling Successful GFP-Like Proteins with Alanine as the Third Chromophore-Forming Residue.
Muslinkina L, Roldán-Salgado A, Gaytán P, Juárez-González VR, Rudiño E, Pletneva N, Pletnev V, Dauter Z, Pletnev S. Muslinkina L, et al. Among authors: gaytan p. J Mol Biol. 2019 Mar 29;431(7):1397-1408. doi: 10.1016/j.jmb.2019.02.013. Epub 2019 Feb 22. J Mol Biol. 2019. PMID: 30797856 Free PMC article.
The mKate fluorescent protein expressed by Leishmania mexicana modifies the parasite immunopathogenicity in BALB/c mice.
Diupotex M, Martínez-Salazar MB, Escalona-Montaño AR, Zamora-Chimal J, Salaiza-Suazo N, Ruiz-Remigio A, Roldán-Salgado A, Aguirre-García MM, Martínez-Calvillo S, Gaytán P, Becker I. Diupotex M, et al. Among authors: gaytan p. Parasite Immunol. 2019 Feb;41(2):e12608. doi: 10.1111/pim.12608. Epub 2018 Dec 27. Parasite Immunol. 2019. PMID: 30500992 Free article.
Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly.
Cárcamo E, Roldán-Salgado A, Osuna J, Bello-Sanmartin I, Yáñez JA, Saab-Rincón G, Viadiu H, Gaytán P. Cárcamo E, et al. Among authors: gaytan p. ACS Omega. 2017 Jul 31;2(7):3183-3191. doi: 10.1021/acsomega.7b00508. Epub 2017 Jul 5. ACS Omega. 2017. PMID: 30023688 Free PMC article.
TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons.
Gaytán P, Contreras-Zambrano C, Ortiz-Alvarado M, Morales-Pablos A, Yáñez J. Gaytán P, et al. Nucleic Acids Res. 2009 Oct;37(18):e125. doi: 10.1093/nar/gkp602. Epub 2009 Sep 25. Nucleic Acids Res. 2009. PMID: 19783828 Free PMC article.
A Codon Deletion at the Beginning of Green Fluorescent Protein Genes Enhances Protein Expression.
Rodríguez-Mejía JL, Roldán-Salgado A, Osuna J, Merino E, Gaytán P. Rodríguez-Mejía JL, et al. J Mol Microbiol Biotechnol. 2017;27(1):1-10. doi: 10.1159/000448786. Epub 2016 Nov 8. J Mol Microbiol Biotechnol. 2017. PMID: 27820932
CiPerGenesis, A Mutagenesis Approach that Produces Small Libraries of Circularly Permuted Proteins Randomly Opened at a Focused Region: Testing on the Green Fluorescent Protein.
Gaytán P, Roldán-Salgado A, Yáñez JA, Morales-Arrieta S, Juárez-González VR. Gaytán P, et al. ACS Comb Sci. 2018 Jul 9;20(7):400-413. doi: 10.1021/acscombsci.7b00152. Epub 2018 Jun 12. ACS Comb Sci. 2018. PMID: 29812897
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