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Efficient selection for high-expression transfectants with a novel eukaryotic vector.
Niwa H, Yamamura K, Miyazaki J. Niwa H, et al. Gene. 1991 Dec 15;108(2):193-9. doi: 10.1016/0378-1119(91)90434-d. Gene. 1991. PMID: 1660837
We have developed a new expression vector which allows efficient selection for transfectants that express foreign genes at high levels. The vector is composed of a ubiquitously strong promoter based on the beta-actin promoter, a 69% subregion of the bovine papilloma virus …
We have developed a new expression vector which allows efficient selection for transfectants that express foreign genes at high level …
Selection for Tn10 tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif.
Wissmann A, Wray LV Jr, Somaggio U, Baumeister R, Geissendörfer M, Hillen W. Wissmann A, et al. Genetics. 1991 Jun;128(2):225-32. Genetics. 1991. PMID: 1649065 Free PMC article.
In this strain Tet repressor blocks expression of lacI and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be selected by growth on galactose as the sole carbon source and screened for the beta-galactosidase phenotype. ...These mutations ar …
In this strain Tet repressor blocks expression of lacI and lacZ. This leads to derepression of a lacPO controlled galK gene. The stra …