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Generation of functional eggs and sperm from cryopreserved whole testes.
Lee S, Iwasaki Y, Shikina S, Yoshizaki G. Lee S, et al. Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1640-5. doi: 10.1073/pnas.1218468110. Epub 2013 Jan 14. Proc Natl Acad Sci U S A. 2013. PMID: 23319620 Free PMC article.
Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodology for cryopreservation of fish eggs and embryos has not yet been developed. The present study established a methodology capa …
Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodol …
Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok).
Lee S, Yoshizaki G. Lee S, et al. Cryobiology. 2016 Apr;72(2):165-8. doi: 10.1016/j.cryobiol.2016.01.004. Epub 2016 Jan 28. Cryobiology. 2016. PMID: 26827783 Free article.
Because of the lack of cryopreservation techniques for fish eggs and embryos, cryopreservation of fish spermatogonia and subsequent generation of eggs and sperm would be an exit strategy for the long-term preservation of genetic resources …
Because of the lack of cryopreservation techniques for fish eggs and embryos, cryopreservation of fish spermatogonia an …
Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells.
Lee S, Katayama N, Yoshizaki G. Lee S, et al. Biochem Biophys Res Commun. 2016 Sep 23;478(3):1478-83. doi: 10.1016/j.bbrc.2016.08.156. Epub 2016 Aug 28. Biochem Biophys Res Commun. 2016. PMID: 27581197
We previously developed an alternative method to derive functional eggs and sperm from cryopreserved whole testis by transplanting testicular cells into female and male recipients. ...Inseminating the resultant eggs and sperm …
We previously developed an alternative method to derive functional eggs and sperm from cryopreserved whole …
Vitrification of medaka whole testis with a trehalose-containing solution and production of medaka individuals derived from the vitrified cells.
Seki S, Yano M, Higashiya M, Oikawa T, Yamazaki W, Yoshizaki G. Seki S, et al. Cryobiology. 2024 Sep;116:104936. doi: 10.1016/j.cryobiol.2024.104936. Epub 2024 Jul 9. Cryobiology. 2024. PMID: 38942069 Free article.
The cryopreservation of teleost eggs and embryos remains challenging, and there are no previous reports that demonstrate successful cryopreservation in medaka (Oryzias latipes). We have reported egg and sperm production, followed by the generation …
The cryopreservation of teleost eggs and embryos remains challenging, and there are no previous reports that demonstrate succe …