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Page 1
Generation of transgenic axolotls (Ambystoma mexicanum).
Khattak S, Richter T, Tanaka EM. Khattak S, et al. Cold Spring Harb Protoc. 2009 Aug;2009(8):pdb.prot5264. doi: 10.1101/pdb.prot5264. Cold Spring Harb Protoc. 2009. PMID: 20147240 No abstract available.
Baculovirus Production and Infection in Axolotls.
Murawala P, Oliveira CR, Okulski H, Yun MH, Tanaka EM. Murawala P, et al. Methods Mol Biol. 2023;2562:369-387. doi: 10.1007/978-1-0716-2659-7_24. Methods Mol Biol. 2023. PMID: 36272088 Free PMC article.
Salamanders have served as an excellent model for developmental and tissue regeneration studies. While transgenic approaches are available for various salamander species, their long generation time and expensive maintenance have driven the development of alternative …
Salamanders have served as an excellent model for developmental and tissue regeneration studies. While transgenic approaches are avai …
The use of transgenics in the laboratory axolotl.
Tilley L, Papadopoulos SC, Pende M, Fei JF, Murawala P. Tilley L, et al. Dev Dyn. 2022 Jun;251(6):942-956. doi: 10.1002/dvdy.357. Epub 2021 May 13. Dev Dyn. 2022. PMID: 33949035 Free PMC article. Review.
The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide variety of model organisms. ...Compared to other widely used model organisms such as mice, zebrafish and fruit flies, there are only a few tr …
The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide vari …
Ependymoglial cells are critical for cortex regeneration in axolotls.
Fu S, Zeng YY, Peng C, Wang L, Feng Y, Wang K, Liu Y, Fei JF. Fu S, et al. Nat Commun. 2026 Jan 20;17(1):1827. doi: 10.1038/s41467-026-68538-6. Nat Commun. 2026. PMID: 41559073 Free PMC article.
Developing precise targeted cell ablation in the axolotl (Ambystoma mexicanum) is crucial for elucidating the roles or interactions of specific cell types in regeneration and modeling diseases. Here we establish a Nitroreductase (NTR)-based inducible cell abl …
Developing precise targeted cell ablation in the axolotl (Ambystoma mexicanum) is crucial for elucidating the roles or …
Transgenesis in axolotl (Ambystoma mexicanum).
Khattak S, Tanaka EM. Khattak S, et al. Methods Mol Biol. 2015;1290:269-77. doi: 10.1007/978-1-4939-2495-0_21. Methods Mol Biol. 2015. PMID: 25740493
Transgenic animals have been indispensable in elucidating and deciphering mechanisms underlying various biological phenomena. ...Here, we describe the generation of germline transgenic axolotls (Ambystoma mexicanum) using the I-SceI megan …
Transgenic animals have been indispensable in elucidating and deciphering mechanisms underlying various biological phenomena. ...Here …
Pseudotyped retroviruses for infecting axolotl.
Kuo TH, Whited JL. Kuo TH, et al. Methods Mol Biol. 2015;1290:127-40. doi: 10.1007/978-1-4939-2495-0_10. Methods Mol Biol. 2015. PMID: 25740482
The ability to introduce DNA elements into host cells and analyze the effects has revolutionized modern biology. Here we describe a protocol to generate Moloney murine leukemia virus (MMLV)-based, replication-incompetent pseudotyped retrovirus capable of infecting axolo …
The ability to introduce DNA elements into host cells and analyze the effects has revolutionized modern biology. Here we describe a protocol …
Visualization of retinoic acid signaling in transgenic axolotls during limb development and regeneration.
Monaghan JR, Maden M. Monaghan JR, et al. Dev Biol. 2012 Aug 1;368(1):63-75. doi: 10.1016/j.ydbio.2012.05.015. Epub 2012 May 22. Dev Biol. 2012. PMID: 22627291 Free PMC article.
To facilitate the study of RA signaling during development and then during regeneration of the same structure we have turned to the axolotl, the master of vertebrate regeneration, and generated transgenic animals that fluorescently report RA signaling in vivo …
To facilitate the study of RA signaling during development and then during regeneration of the same structure we have turned to the axolo …
Establishment of a Practical Sperm Cryopreservation Pathway for the Axolotl (Ambystoma mexicanum): A Community-Level Approach to Germplasm Repository Development.
Coxe N, Liu Y, Arregui L, Upton R, Bodenstein S, Voss SR, Gutierrez-Wing MT, Tiersch TR. Coxe N, et al. Animals (Basel). 2024 Jan 8;14(2):206. doi: 10.3390/ani14020206. Animals (Basel). 2024. PMID: 38254376 Free PMC article.
The axolotl (Ambystoma mexicanum) draws great attention around the world for its importance as a biomedical research model, but housing and maintaining live animals is increasingly expensive and risky as new transgenic lines are developed. ...This work …
The axolotl (Ambystoma mexicanum) draws great attention around the world for its importance as a biomedical research mo …
A constitutively expressed fluorescent ubiquitination-based cell-cycle indicator (FUCCI) in axolotls for studying tissue regeneration.
Duerr TJ, Jeon EK, Wells KM, Villanueva A, Seifert AW, McCusker CD, Monaghan JR. Duerr TJ, et al. Development. 2022 Mar 15;149(6):dev199637. doi: 10.1242/dev.199637. Epub 2022 Mar 17. Development. 2022. PMID: 35266986 Free PMC article.
Regulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, the axolotl (Ambystoma mexicanum) regenerates missing structures through an accumulation of proliferating cells known as …
Regulation of cell cycle progression is essential for cell proliferation during regeneration following injury. After appendage amputation, t …
Is salamander limb regeneration really perfect? Anatomical and morphogenetic analysis of forelimb muscle regeneration in GFP-transgenic axolotls as a basis for regenerative, developmental, and evolutionary studies.
Diogo R, Nacu E, Tanaka EM. Diogo R, et al. Anat Rec (Hoboken). 2014 Jun;297(6):1076-89. doi: 10.1002/ar.22906. Epub 2014 Apr 2. Anat Rec (Hoboken). 2014. PMID: 24692358 Free article.
The axolotl Ambystoma mexicanum is one of the most commonly used model organisms in developmental and regenerative studies because it can reconstitute what is believed to be a completely normal anatomical and functional forelimb/hindlimb after amputation. ... …
The axolotl Ambystoma mexicanum is one of the most commonly used model organisms in developmental and regenerative stud …
16 results