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Catching a DUB in the act: novel ubiquitin-based active site directed probes.
Ekkebus R, Flierman D, Geurink PP, Ovaa H. Ekkebus R, et al. Among authors: geurink pp. Curr Opin Chem Biol. 2014 Dec;23:63-70. doi: 10.1016/j.cbpa.2014.10.005. Curr Opin Chem Biol. 2014. PMID: 25461387 Free PMC article. Review.
The differential modulation of USP activity by internal regulatory domains, interactors and eight ubiquitin chain types.
Faesen AC, Luna-Vargas MP, Geurink PP, Clerici M, Merkx R, van Dijk WJ, Hameed DS, El Oualid F, Ovaa H, Sixma TK. Faesen AC, et al. Among authors: geurink pp. Chem Biol. 2011 Dec 23;18(12):1550-61. doi: 10.1016/j.chembiol.2011.10.017. Chem Biol. 2011. PMID: 22195557
On terminal alkynes that can react with active-site cysteine nucleophiles in proteases.
Ekkebus R, van Kasteren SI, Kulathu Y, Scholten A, Berlin I, Geurink PP, de Jong A, Goerdayal S, Neefjes J, Heck AJ, Komander D, Ovaa H. Ekkebus R, et al. Among authors: geurink pp. J Am Chem Soc. 2013 Feb 27;135(8):2867-70. doi: 10.1021/ja309802n. Epub 2013 Feb 15. J Am Chem Soc. 2013. PMID: 23387960 Free PMC article.
Development of Diubiquitin-Based FRET Probes To Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes.
Geurink PP, van Tol BD, van Dalen D, Brundel PJ, Mevissen TE, Pruneda JN, Elliott PR, van Tilburg GB, Komander D, Ovaa H. Geurink PP, et al. Chembiochem. 2016 May 3;17(9):816-20. doi: 10.1002/cbic.201600017. Epub 2016 Mar 21. Chembiochem. 2016. PMID: 26996281 Free PMC article.
The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases.
Pruneda JN, Durkin CH, Geurink PP, Ovaa H, Santhanam B, Holden DW, Komander D. Pruneda JN, et al. Among authors: geurink pp. Mol Cell. 2016 Jul 21;63(2):261-276. doi: 10.1016/j.molcel.2016.06.015. Epub 2016 Jul 14. Mol Cell. 2016. PMID: 27425412 Free PMC article.
Identification and characterization of diverse OTU deubiquitinases in bacteria.
Schubert AF, Nguyen JV, Franklin TG, Geurink PP, Roberts CG, Sanderson DJ, Miller LN, Ovaa H, Hofmann K, Pruneda JN, Komander D. Schubert AF, et al. Among authors: geurink pp. EMBO J. 2020 Aug 3;39(15):e105127. doi: 10.15252/embj.2020105127. Epub 2020 Jun 22. EMBO J. 2020. PMID: 32567101 Free PMC article.
Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity.
Shin D, Mukherjee R, Grewe D, Bojkova D, Baek K, Bhattacharya A, Schulz L, Widera M, Mehdipour AR, Tascher G, Geurink PP, Wilhelm A, van der Heden van Noort GJ, Ovaa H, Müller S, Knobeloch KP, Rajalingam K, Schulman BA, Cinatl J, Hummer G, Ciesek S, Dikic I. Shin D, et al. Among authors: geurink pp. Nature. 2020 Nov;587(7835):657-662. doi: 10.1038/s41586-020-2601-5. Epub 2020 Jul 29. Nature. 2020. PMID: 32726803
Non-hydrolyzable Diubiquitin Probes Reveal Linkage-Specific Reactivity of Deubiquitylating Enzymes Mediated by S2 Pockets.
Flierman D, van der Heden van Noort GJ, Ekkebus R, Geurink PP, Mevissen TE, Hospenthal MK, Komander D, Ovaa H. Flierman D, et al. Among authors: geurink pp. Cell Chem Biol. 2016 Apr 21;23(4):472-82. doi: 10.1016/j.chembiol.2016.03.009. Epub 2016 Apr 7. Cell Chem Biol. 2016. PMID: 27066941 Free PMC article.
A MALDI-TOF Approach to Ubiquitin Ligase Activity.
van Tol BDM, Geurink PP, Ovaa H. van Tol BDM, et al. Among authors: geurink pp. Cell Chem Biol. 2018 Sep 20;25(9):1053-1055. doi: 10.1016/j.chembiol.2018.09.002. Cell Chem Biol. 2018. PMID: 30241600
Poly(ethylene glycol)-based stable isotope labeling reagents for the quantitative analysis of low molecular weight metabolites by LC-MS.
Abello N, Geurink PP, van der Toorn M, van Oosterhout AJ, Lugtenburg J, van der Marel GA, Kerstjens HA, Postma DS, Overkleeft HS, Bischoff R. Abello N, et al. Among authors: geurink pp. Anal Chem. 2008 Dec 1;80(23):9171-80. doi: 10.1021/ac801215c. Anal Chem. 2008. PMID: 18954088
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