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Enzymatic assembly of overlapping DNA fragments.
Gibson DG. Gibson DG. Methods Enzymol. 2011;498:349-61. doi: 10.1016/B978-0-12-385120-8.00015-2. Methods Enzymol. 2011. PMID: 21601685 Free PMC article.
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.
Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, Smith HO. Gibson DG, et al. Science. 2008 Feb 29;319(5867):1215-20. doi: 10.1126/science.1151721. Epub 2008 Jan 24. Science. 2008. PMID: 18218864
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.
Gibson DG, Benders GA, Axelrod KC, Zaveri J, Algire MA, Moodie M, Montague MG, Venter JC, Smith HO, Hutchison CA 3rd. Gibson DG, et al. Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20404-9. doi: 10.1073/pnas.0811011106. Epub 2008 Dec 10. Proc Natl Acad Sci U S A. 2008. PMID: 19073939 Free PMC article.
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Gibson DG, et al. Nat Methods. 2009 May;6(5):343-5. doi: 10.1038/nmeth.1318. Epub 2009 Apr 12. Nat Methods. 2009. PMID: 19363495
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI. Lartigue C, et al. Among authors: gibson dg. Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20. Science. 2009. PMID: 19696314
Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides.
Gibson DG. Gibson DG. Nucleic Acids Res. 2009 Nov;37(20):6984-90. doi: 10.1093/nar/gkp687. Epub 2009 Sep 10. Nucleic Acids Res. 2009. PMID: 19745056 Free PMC article.
Cloning whole bacterial genomes in yeast.
Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA 3rd. Benders GA, et al. Among authors: gibson dg. Nucleic Acids Res. 2010 May;38(8):2558-69. doi: 10.1093/nar/gkq119. Epub 2010 Mar 7. Nucleic Acids Res. 2010. PMID: 20211840 Free PMC article.
Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC. Gibson DG, et al. Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20. Science. 2010. PMID: 20488990
Chemical synthesis of the mouse mitochondrial genome.
Gibson DG, Smith HO, Hutchison CA 3rd, Venter JC, Merryman C. Gibson DG, et al. Nat Methods. 2010 Nov;7(11):901-3. doi: 10.1038/nmeth.1515. Epub 2010 Oct 10. Nat Methods. 2010. PMID: 20935651
Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies.
Noskov VN, Chuang RY, Gibson DG, Leem SH, Larionov V, Kouprina N. Noskov VN, et al. Among authors: gibson dg. Nat Protoc. 2011 Jan;6(1):89-96. doi: 10.1038/nprot.2010.174. Epub 2010 Dec 23. Nat Protoc. 2011. PMID: 21212778 Free PMC article.
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