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Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.
Gietz RD, Schiestl RH. Gietz RD, et al. Yeast. 1991 Apr;7(3):253-63. doi: 10.1002/yea.320070307. Yeast. 1991. PMID: 1882550
The highly efficient yeast lithium acetate transformation protocol of Schiestl and Gietz (1989) was tested for its applicability to some of the most important needs of current yeast molecular biology. ...
The highly efficient yeast lithium acetate transformation protocol of Schiestl and Gietz (1989) was tested for its applicability to s …
Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.
Gietz RD, Schiestl RH, Willems AR, Woods RA. Gietz RD, et al. Yeast. 1995 Apr 15;11(4):355-60. doi: 10.1002/yea.320110408. Yeast. 1995. PMID: 7785336
An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants/micrograms plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mech …
An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants …
Genetic transformation of yeast.
Gietz RD, Woods RA. Gietz RD, et al. Biotechniques. 2001 Apr;30(4):816-20, 822-6, 828 passim. doi: 10.2144/01304rv02. Biotechniques. 2001. PMID: 11314265 Review.
High-efficiency transformation of plasmid DNA into yeast.
Woods RA, Gietz RD. Woods RA, et al. Methods Mol Biol. 2001;177:85-97. doi: 10.1385/1-59259-210-4:085. Methods Mol Biol. 2001. PMID: 11530617 No abstract available.
Rapid screening of plasmid DNA by direct sequencing from bacterial colonies.
Gladden JG, Ray R, Gietz RD, Mowat MR. Gladden JG, et al. Biotechniques. 2000 Sep;29(3):436-8. doi: 10.2144/00293bm06. Biotechniques. 2000. PMID: 10997254 No abstract available.
Screening for protein-protein interactions in the yeast two-hybrid system.
Gietz RD, Woods RA. Gietz RD, et al. Methods Mol Biol. 2002;185:471-86. doi: 10.1385/1-59259-241-4:471. Methods Mol Biol. 2002. PMID: 11769011 Review. No abstract available.
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