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Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.
Gietz RD, Schiestl RH. Gietz RD, et al. Yeast. 1991 Apr;7(3):253-63. doi: 10.1002/yea.320070307. Yeast. 1991. PMID: 1882550
The highly efficient yeast lithium acetate transformation protocol of Schiestl and Gietz (1989) was tested for its applicability to some of the most important needs of current yeast molecular biology. ...
The highly efficient yeast lithium acetate transformation protocol of Schiestl and Gietz (1989) was tested for its applicability to s …
Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.
Gietz RD, Schiestl RH, Willems AR, Woods RA. Gietz RD, et al. Yeast. 1995 Apr 15;11(4):355-60. doi: 10.1002/yea.320110408. Yeast. 1995. PMID: 7785336
An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants/micrograms plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mech …
An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants …
Genetic transformation of yeast.
Gietz RD, Woods RA. Gietz RD, et al. Biotechniques. 2001 Apr;30(4):816-20, 822-6, 828 passim. doi: 10.2144/01304rv02. Biotechniques. 2001. PMID: 11314265 Review.
HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain.
Kalchman MA, Koide HB, McCutcheon K, Graham RK, Nichol K, Nishiyama K, Kazemi-Esfarjani P, Lynn FC, Wellington C, Metzler M, Goldberg YP, Kanazawa I, Gietz RD, Hayden MR. Kalchman MA, et al. Nat Genet. 1997 May;16(1):44-53. doi: 10.1038/ng0597-44. Nat Genet. 1997. PMID: 9140394
Production of digoxigenin-labelled RNA probes and the detection of cytokine mRNA in rat spleen and brain by in situ hybridization.
Meltzer JC, Sanders V, Grimm PC, Stern E, Rivier C, Lee S, Rennie SL, Gietz RD, Hole AK, Watson PH, Greenberg AH, Nance DM. Meltzer JC, et al. Brain Res Brain Res Protoc. 1998 Jun;2(4):339-51. doi: 10.1016/s1385-299x(98)00010-5. Brain Res Brain Res Protoc. 1998. PMID: 9630715
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