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Purification and characterization of N-glycosylation mutant mouse and human P-glycoproteins expressed in Pichia pastoris cells.
Arch Biochem Biophys. 2001 Apr 1;388(1):171-7. doi: 10.1006/abbi.2001.2299.
Arch Biochem Biophys. 2001.
PMID: 11361134
Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues.
Tombline G, Bartholomew LA, Tyndall GA, Gimi K, Urbatsch IL, Senior AE.
Tombline G, et al. Among authors: gimi k.
J Biol Chem. 2004 Nov 5;279(45):46518-26. doi: 10.1074/jbc.M408052200. Epub 2004 Aug 23.
J Biol Chem. 2004.
PMID: 15326176
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Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.
Lerner-Marmarosh N, Gimi K, Urbatsch IL, Gros P, Senior AE.
Lerner-Marmarosh N, et al. Among authors: gimi k.
J Biol Chem. 1999 Dec 3;274(49):34711-8. doi: 10.1074/jbc.274.49.34711.
J Biol Chem. 1999.
PMID: 10574938
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Conserved walker A Ser residues in the catalytic sites of P-glycoprotein are critical for catalysis and involved primarily at the transition state step.
Urbatsch IL, Gimi K, Wilke-Mounts S, Senior AE.
Urbatsch IL, et al. Among authors: gimi k.
J Biol Chem. 2000 Aug 11;275(32):25031-8. doi: 10.1074/jbc.M003962200.
J Biol Chem. 2000.
PMID: 10831598
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Investigation of the role of glutamine-471 and glutamine-1114 in the two catalytic sites of P-glycoprotein.
Urbatsch IL, Gimi K, Wilke-Mounts S, Senior AE.
Urbatsch IL, et al. Among authors: gimi k.
Biochemistry. 2000 Oct 3;39(39):11921-7. doi: 10.1021/bi001220s.
Biochemistry. 2000.
PMID: 11009605
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Cysteines 431 and 1074 are responsible for inhibitory disulfide cross-linking between the two nucleotide-binding sites in human P-glycoprotein.
Urbatsch IL, Gimi K, Wilke-Mounts S, Lerner-Marmarosh N, Rousseau ME, Gros P, Senior AE.
Urbatsch IL, et al. Among authors: gimi k.
J Biol Chem. 2001 Jul 20;276(29):26980-7. doi: 10.1074/jbc.M010829200. Epub 2001 May 16.
J Biol Chem. 2001.
PMID: 11356825
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Synergy between conserved ABC signature Ser residues in P-glycoprotein catalysis.
Tombline G, Bartholomew L, Gimi K, Tyndall GA, Senior AE.
Tombline G, et al. Among authors: gimi k.
J Biol Chem. 2004 Feb 13;279(7):5363-73. doi: 10.1074/jbc.M311964200. Epub 2003 Nov 24.
J Biol Chem. 2004.
PMID: 14638679
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