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Shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of GpAp versus GpA in barnase.
Giraldo J, De Maria L, Wodak SJ. Giraldo J, et al. Proteins. 2004 Aug 1;56(2):261-76. doi: 10.1002/prot.20137. Proteins. 2004. PMID: 15211510
The increase in K(M), caused by the presence of additional non-productive binding modes for GpA, should however be counterbalanced by the propensity of free GpA to adopt folded conformations in solution, which are unable to bind the enzyme and by the tighter binding of GpAp (G
The increase in K(M), caused by the presence of additional non-productive binding modes for GpA, should however be counterbalanced by the pr …
Effect of nucleotide substrate binding on the pKa of catalytic residues in barnase.
Gordon-Beresford RM, Van Belle D, Giraldo J, Wodak SJ. Gordon-Beresford RM, et al. Proteins. 1996 Jun;25(2):180-94. doi: 10.1002/(SICI)1097-0134(199606)25:2<180::AID-PROT4>3.0.CO;2-M. Proteins. 1996. PMID: 8811734
Selective Protonation of Acidic Residues Triggers Opsin Activation.
Lans I, Dalton JA, Giraldo J. Lans I, et al. J Phys Chem B. 2015 Jul 30;119(30):9510-9. doi: 10.1021/acs.jpcb.5b01908. Epub 2015 Jul 17. J Phys Chem B. 2015. PMID: 26140747
Quantifying conformational changes in GPCRs: glimpse of a common functional mechanism.
Dalton JA, Lans I, Giraldo J. Dalton JA, et al. BMC Bioinformatics. 2015 Apr 23;16(1):124. doi: 10.1186/s12859-015-0567-3. BMC Bioinformatics. 2015. PMID: 25902715 Free PMC article.
Modeling the binding and function of metabotropic glutamate receptors.
Rovira X, Roche D, Serra J, Kniazeff J, Pin JP, Giraldo J. Rovira X, et al. J Pharmacol Exp Ther. 2008 May;325(2):443-56. doi: 10.1124/jpet.107.133967. Epub 2008 Feb 20. J Pharmacol Exp Ther. 2008. PMID: 18287211
The first one reports a negative cooperativity in agonist binding (J Biol Chem 279:35526-35534, 2004), whereas the other indicates a positive cooperativity in agonist-mediated response (Nat Struct Mol Biol 11:706-713, 2004). ...
The first one reports a negative cooperativity in agonist binding (J Biol Chem 279:35526-35534, 2004), whereas the other indicates a …
Agonist induction, conformational selection, and mutant receptors.
Giraldo J. Giraldo J. FEBS Lett. 2004 Jan 2;556(1-3):13-8. doi: 10.1016/s0014-5793(03)01404-2. FEBS Lett. 2004. PMID: 14706818
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