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Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals.
Prasad R, De Wergifosse P, Goffeau A, Balzi E. Prasad R, et al. Curr Genet. 1995 Mar;27(4):320-9. doi: 10.1007/BF00352101. Curr Genet. 1995. PMID: 7614555
By functional complementation of a PDR5 null mutant of Saccharomyces cerevisiae, we have cloned and sequenced the multidrug-resistance gene CDR1 of Candida albicans. ...CDR1 encodes a 1501-residue protein of 169.9 kDa whose predicted structural organization is chara …
By functional complementation of a PDR5 null mutant of Saccharomyces cerevisiae, we have cloned and sequenced the multidrug-resistanc …
The multidrug resistance gene PDR1 from Saccharomyces cerevisiae.
Balzi E, Chen W, Ulaszewski S, Capieaux E, Goffeau A. Balzi E, et al. J Biol Chem. 1987 Dec 15;262(35):16871-9. J Biol Chem. 1987. PMID: 3316228
A RNA molecule of 3.5 kilobases was identified as the PDR1 transcript. The nucleotide sequence of the complementing DNA fragment contained a 3192-nucleotide open reading frame. ...A model is proposed of a general transcriptional control by PDR1 of seve
A RNA molecule of 3.5 kilobases was identified as the PDR1 transcript. The nucleotide sequence of the complementing DNA fragment cont
The pma1 and pma2 H(+)-ATPases from Schizosaccharomyces pombe are functionally interchangeable.
Ghislain M, Goffeau A. Ghislain M, et al. J Biol Chem. 1991 Sep 25;266(27):18276-9. J Biol Chem. 1991. PMID: 1833395
The pma2 gene of Schizosaccharomyces pombe codes for a polypeptide having a predicted Mr of 110,126 and which is 79% identical to the plasma membrane H(+)-ATPase encoded by the pma1 gene. ...In a pma1-1 mutant strain whose ATPase activity is insensitive to va …
The pma2 gene of Schizosaccharomyces pombe codes for a polypeptide having a predicted Mr of 110,126 and which is 79% identical …
Identification and characterization of SNQ2, a new multidrug ATP binding cassette transporter of the yeast plasma membrane.
Decottignies A, Lambert L, Catty P, Degand H, Epping EA, Moye-Rowley WS, Balzi E, Goffeau A. Decottignies A, et al. J Biol Chem. 1995 Jul 28;270(30):18150-7. doi: 10.1074/jbc.270.30.18150. J Biol Chem. 1995. PMID: 7629127
In a plasma membrane-enriched fraction from a pdr1 mutant, the SNQ2 protein is found in the 160-kDa over-expressed band, together with PDR5. ...The enzyme shows a nucleoside triphosphatase activity that differs biochemically from that of PDR5 (Decottignies, …
In a plasma membrane-enriched fraction from a pdr1 mutant, the SNQ2 protein is found in the 160-kDa over-expressed band, toget …
The uses of genome-wide yeast mutant collections.
Scherens B, Goffeau A. Scherens B, et al. Genome Biol. 2004;5(7):229. doi: 10.1186/gb-2004-5-7-229. Epub 2004 Jun 25. Genome Biol. 2004. PMID: 15239820 Free PMC article. Review.
Novel transporters from hemiascomycete yeasts.
De Hertogh B, Talla E, Tekaia F, Beyne E, Sherman D, Baret PV, Dujon B, Goffeau A. De Hertogh B, et al. J Mol Microbiol Biotechnol. 2003;6(1):19-28. doi: 10.1159/000073405. J Mol Microbiol Biotechnol. 2003. PMID: 14593250
We screened nearly one thousand random sequenced targets obtained by partial sequencing of 13 hemiascomycete genomes identified by higher amino acid sequence similarity to a non-Saccharomyces cerevisiae protein than to a S. ...
We screened nearly one thousand random sequenced targets obtained by partial sequencing of 13 hemiascomycete genomes identified by higher am …
Phylogenetic classification of transporters and other membrane proteins from Saccharomyces cerevisiae.
De Hertogh B, Carvajal E, Talla E, Dujon B, Baret P, Goffeau A. De Hertogh B, et al. Funct Integr Genomics. 2002 Sep;2(4-5):154-70. doi: 10.1007/s10142-002-0060-8. Epub 2002 Jun 5. Funct Integr Genomics. 2002. PMID: 12192589 Review.
On the basis of functional and phylogenetic criteria, we have identified a total of 229 subfamilies and 111 singletons predicted to carry out transport or other membrane functions in Saccharomyces cerevisiae. We have extended the Transporter Classification (TC) and created …
On the basis of functional and phylogenetic criteria, we have identified a total of 229 subfamilies and 111 singletons predicted to c …
Regulation of pleiotropic drug resistance in yeast.
Kolaczkowska A, Goffeau A. Kolaczkowska A, et al. Drug Resist Updat. 1999 Dec;2(6):403-414. doi: 10.1054/drup.1999.0113. Drug Resist Updat. 1999. PMID: 11498356
Understanding the molecular basis leading to multiple drug resistance is a prerequisite for the development of new antifungal therapeutics. ...
Understanding the molecular basis leading to multiple drug resistance is a prerequisite for the development of new antifungal therape …
Characterization of a hyperthermophilic P-type ATPase from Methanococcus jannaschii expressed in yeast.
Morsomme P, Chami M, Marco S, Nader J, Ketchum KA, Goffeau A, Rigaud JL. Morsomme P, et al. J Biol Chem. 2002 Aug 16;277(33):29608-16. doi: 10.1074/jbc.M203871200. Epub 2002 Jun 4. J Biol Chem. 2002. PMID: 12048206
An efficient heterologous expression system was developed in Saccharomyces cerevisiae and a four-step purification protocol, using n-dodecyl beta-d-maltoside, led to a homogeneous detergent-solubilized protein fraction with a yield of over 2 mg of protein per …
An efficient heterologous expression system was developed in Saccharomyces cerevisiae and a four-step purification protocol, using n- …
Emergence of species-specific transporters during evolution of the hemiascomycete phylum.
De Hertogh B, Hancy F, Goffeau A, Baret PV. De Hertogh B, et al. Genetics. 2006 Feb;172(2):771-81. doi: 10.1534/genetics.105.046813. Epub 2005 Aug 22. Genetics. 2006. PMID: 16118182 Free PMC article.
The use of nonambiguous functional and phylogenetic criteria derived from the TCDB classification system has allowed the identification within the Hemiascomycete phylum of 97 small phylogenetic transporter subfamilies comprising a total of 355 transporters submitted to fou …
The use of nonambiguous functional and phylogenetic criteria derived from the TCDB classification system has allowed the identification with …
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