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A pilot project to generate affinity reagents to human proteins.
Uhlen M, Gräslund S, Sundström M. Uhlen M, et al. Nat Methods. 2008 Oct;5(10):854-5. doi: 10.1038/nmeth1008-854. Nat Methods. 2008. PMID: 18825127 No abstract available.
A roadmap to generate renewable protein binders to the human proteome.
Colwill K; Renewable Protein Binder Working Group, Gräslund S. Colwill K, et al. Nat Methods. 2011 May 15;8(7):551-8. doi: 10.1038/nmeth.1607. Nat Methods. 2011. PMID: 21572409
Protein production and purification.
Structural Genomics Consortium; China Structural Genomics Consortium; Northeast Structural Genomics Consortium, Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC. Structural Genomics Consortium, et al. Nat Methods. 2008 Feb;5(2):135-46. doi: 10.1038/nmeth.f.202. Nat Methods. 2008. PMID: 18235434 Free PMC article. Review.
In situ proteolysis for protein crystallization and structure determination.
Dong A, Xu X, Edwards AM; Midwest Center for Structural Genomics; Structural Genomics Consortium, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Gräslund S, Hammerström M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H. Dong A, et al. Nat Methods. 2007 Dec;4(12):1019-21. doi: 10.1038/nmeth1118. Epub 2007 Nov 4. Nat Methods. 2007. PMID: 17982461 Free PMC article.
Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(+).
Cameron WD, Bui CV, Hutchinson A, Loppnau P, Gräslund S, Rocheleau JV. Cameron WD, et al. Among authors: graslund s. Nat Methods. 2016 Apr;13(4):352-8. doi: 10.1038/nmeth.3764. Epub 2016 Feb 15. Nat Methods. 2016. PMID: 26878383
Towards proteome scale antibody selections using phage display.
Mersmann M, Meier D, Mersmann J, Helmsing S, Nilsson P, Gräslund S; Structural Genomics Consortium, Colwill K, Hust M, Dübel S. Mersmann M, et al. N Biotechnol. 2010 May 31;27(2):118-28. doi: 10.1016/j.nbt.2009.10.007. Epub 2009 Oct 31. N Biotechnol. 2010. PMID: 19883803
Recombinant renewable polyclonal antibodies.
Ferrara F, D'Angelo S, Gaiotto T, Naranjo L, Tian H, Gräslund S, Dobrovetsky E, Hraber P, Lund-Johansen F, Saragozza S, Sblattero D, Kiss C, Bradbury AR. Ferrara F, et al. MAbs. 2015;7(1):32-41. doi: 10.4161/19420862.2015.989047. MAbs. 2015. PMID: 25530082 Free PMC article.
Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display.
Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J. Pershad K, et al. Protein Eng Des Sel. 2010 Apr;23(4):279-88. doi: 10.1093/protein/gzq003. Epub 2010 Feb 17. Protein Eng Des Sel. 2010. PMID: 20164216 Free PMC article.
Crystal structure of human inosine triphosphatase. Substrate binding and implication of the inosine triphosphatase deficiency mutation P32T.
Stenmark P, Kursula P, Flodin S, Gräslund S, Landry R, Nordlund P, Schüler H. Stenmark P, et al. J Biol Chem. 2007 Feb 2;282(5):3182-7. doi: 10.1074/jbc.M609838200. Epub 2006 Nov 29. J Biol Chem. 2007. PMID: 17138556
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