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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1932 1
1933 2
1940 1
1942 1
1944 5
1945 1
1946 22
1947 15
1948 15
1949 12
1950 7
1951 13
1952 11
1953 14
1954 10
1955 14
1956 14
1957 13
1958 12
1959 16
1960 16
1961 16
1962 14
1963 17
1964 21
1965 18
1966 18
1967 25
1968 31
1969 30
1970 27
1971 28
1972 43
1973 46
1974 46
1975 49
1976 57
1977 54
1978 50
1979 77
1980 56
1981 57
1982 47
1983 60
1984 65
1985 69
1986 79
1987 74
1988 108
1989 120
1990 106
1991 112
1992 124
1993 113
1994 133
1995 117
1996 131
1997 118
1998 136
1999 109
2000 116
2001 132
2002 120
2003 129
2004 183
2005 180
2006 186
2007 164
2008 188
2009 177
2010 192
2011 186
2012 194
2013 225
2014 224
2015 266
2016 254
2017 281
2018 293
2019 299
2020 53
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6,434 results
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Page 1
Touchdown Polymerase Chain Reaction (PCR)
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 29717053
In touchdown PCR the temperature selected for the annealing step is initially set 5°C-10°C higher than the calculated T(m) of the primers. ...In subsequent cycles, the annealing temperature is gradually decreased by a small amount so that by the end of the PCR, the annealing temperature is 2°C-5°C below the calculated T(m) of the primers. ...
In touchdown PCR the temperature selected for the annealing step is initially set 5°C-10°C higher than the calculated T(m) of the pri …
Buffers
Green MR and Sambrook J. Cold Spring Harb Protoc 2018. PMID 29438064
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