Preliminary characterization of a cholinesterase from roots of Bengal gram - Cicer arietinum L

R Gupta; S C Maheshwari

doi:10.1093/pcp/21.8.1675

Preliminary characterization of a cholinesterase from roots of Bengal gram - Cicer arietinum L

Plant Cell Physiol. 1980 Dec;21(8):1675-9. doi: 10.1093/pcp/21.8.1675.

Authors

R Gupta¹, S C Maheshwari¹

Affiliation

¹ Department of Botany, University of Delhi, Delhi-110007, India.

PMID: 25385985
DOI: 10.1093/pcp/21.8.1675

Abstract

A Cholinesterase was isolated and purified (65-fold) from roots of Bengal gram (Cicer arietinum L.) seedlings. It hydrolyzes acetylcholine and acetylthiocholine more readily than propionylthiocholine or butyrylthiocholine. The enzyme has high affinity for acetylthiocholine (Km=150 μm) and is inhibited by animal anticholinesterases, neostigmine and physostigmine, and by Phosfon-D, a plant growth retardant.

Keywords: Acetylcholine; Bengal gram; Cholinesterase; Cicer arietinum; Neostigmine; Phosfon-D; Physostigmine.