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Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts.
Agaton C, Falk R, Höidén Guthenberg I, Göstring L, Uhlén M, Hober S. Agaton C, et al. J Chromatogr A. 2004 Jul 16;1043(1):33-40. doi: 10.1016/j.chroma.2004.06.008. J Chromatogr A. 2004. PMID: 15317410
The results suggest that the protocol can be used in a highly parallel and automated manner to generate mono-specific polyclonal antibodies for large-scale, antibody-based proteomics efforts, i.e. affinity proteomics....
The results suggest that the protocol can be used in a highly parallel and automated manner to generate mono-specific polyclonal antibodies …
Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.
Kronqvist N, Malm M, Göstring L, Gunneriusson E, Nilsson M, Höidén Guthenberg I, Gedda L, Frejd FY, Ståhl S, Löfblom J. Kronqvist N, et al. Among authors: hoiden guthenberg i. Protein Eng Des Sel. 2011 Apr;24(4):385-96. doi: 10.1093/protein/gzq118. Epub 2010 Dec 21. Protein Eng Des Sel. 2011. PMID: 21177282
Directed evolution to low nanomolar affinity of a tumor-targeting epidermal growth factor receptor-binding affibody molecule.
Friedman M, Orlova A, Johansson E, Eriksson TL, Höidén-Guthenberg I, Tolmachev V, Nilsson FY, Ståhl S. Friedman M, et al. J Mol Biol. 2008 Mar 7;376(5):1388-402. doi: 10.1016/j.jmb.2007.12.060. Epub 2008 Jan 4. J Mol Biol. 2008. PMID: 18207161
Affibody-mediated transferrin depletion for proteomics applications.
Grönwall C, Sjöberg A, Ramström M, Höidén-Guthenberg I, Hober S, Jonasson P, Ståhl S. Grönwall C, et al. Biotechnol J. 2007 Nov;2(11):1389-98. doi: 10.1002/biot.200700053. Biotechnol J. 2007. PMID: 17639529
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
Agaton C, Galli J, Höidén Guthenberg I, Janzon L, Hansson M, Asplund A, Brundell E, Lindberg S, Ruthberg I, Wester K, Wurtz D, Höög C, Lundeberg J, Ståhl S, Pontén F, Uhlén M. Agaton C, et al. Mol Cell Proteomics. 2003 Jun;2(6):405-14. doi: 10.1074/mcp.M300022-MCP200. Epub 2003 Jun 9. Mol Cell Proteomics. 2003. PMID: 12796447
Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol.
Rönnmark J, Kampf C, Asplund A, Höidén-Guthenberg I, Wester K, Pontén F, Uhlén M, Nygren PA. Rönnmark J, et al. J Immunol Methods. 2003 Oct 1;281(1-2):149-60. doi: 10.1016/j.jim.2003.06.001. J Immunol Methods. 2003. PMID: 14580889
Site-specifically conjugated anti-HER2 Affibody molecules as one-step reagents for target expression analyses on cells and xenograft samples.
Lundberg E, Höidén-Guthenberg I, Larsson B, Uhlén M, Gräslund T. Lundberg E, et al. J Immunol Methods. 2007 Jan 30;319(1-2):53-63. doi: 10.1016/j.jim.2006.10.013. Epub 2006 Nov 21. J Immunol Methods. 2007. PMID: 17196217
Engineered high-affinity affibody molecules targeting platelet-derived growth factor receptor β in vivo.
Lindborg M, Cortez E, Höidén-Guthenberg I, Gunneriusson E, von Hage E, Syud F, Morrison M, Abrahmsén L, Herne N, Pietras K, Frejd FY. Lindborg M, et al. Among authors: hoiden guthenberg i. J Mol Biol. 2011 Mar 25;407(2):298-315. doi: 10.1016/j.jmb.2011.01.033. Epub 2011 Jan 28. J Mol Biol. 2011. PMID: 21277312
Inhibiting HER3-mediated tumor cell growth with affibody molecules engineered to low picomolar affinity by position-directed error-prone PCR-like diversification.
Malm M, Kronqvist N, Lindberg H, Gudmundsdotter L, Bass T, Frejd FY, Höidén-Guthenberg I, Varasteh Z, Orlova A, Tolmachev V, Ståhl S, Löfblom J. Malm M, et al. Among authors: hoiden guthenberg i. PLoS One. 2013 May 10;8(5):e62791. doi: 10.1371/journal.pone.0062791. Print 2013. PLoS One. 2013. PMID: 23675426 Free PMC article.
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