Temperature increases from 55 to 75 °C in a two-phase biogas reactor result in fundamental alterations within the bacterial and archaeal community structure

Appl Microbiol Biotechnol. 2012 Oct;96(2):565-76. doi: 10.1007/s00253-012-4348-x. Epub 2012 Aug 17.

Abstract

Agricultural biogas plants were operated in most cases below their optimal performance. An increase in the fermentation temperature and a spatial separation of hydrolysis/acetogenesis and methanogenesis are known strategies in improving and stabilizing biogas production. In this study, the dynamic variability of the bacterial and archaeal community was monitored within a two-phase leach bed biogas reactor supplied with rye silage and straw during a stepwise temperature increase from 55 to 75 °C within the leach bed reactor (LBR), using TRFLP analyses. To identify the terminal restriction fragments that were obtained, bacterial and archaeal 16S rRNA gene libraries were constructed. Above 65 °C, the bacterial community structure changed from being Clostridiales-dominated toward being dominated by members of the Bacteroidales, Clostridiales, and Thermotogales orders. Simultaneously, several changes occurred, including a decrease in the total cell count, degradation rate, and biogas yield along with alterations in the intermediate production. A bioaugmentation with compost at 70 °C led to slight improvements in the reactor performance; these did not persist at 75 °C. However, the archaeal community within the downstream anaerobic filter reactor (AF), operated constantly at 55 °C, altered by the temperature increase in the LBR. At an LBR temperature of 55 °C, members of the Methanobacteriales order were prevalent in the AF, whereas at higher LBR temperatures Methanosarcinales prevailed. Altogether, the best performance of this two-phase reactor was achieved at an LBR temperature of below 65 °C, which indicates that this temperature range has a favorable effect on the microbial community responsible for the production of biogas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / classification
  • Archaea / genetics
  • Archaea / isolation & purification*
  • Archaea / metabolism
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Bacteria / metabolism
  • Biodiversity
  • Biofuels / analysis*
  • Biofuels / microbiology
  • Bioreactors / microbiology*
  • Hot Temperature
  • Methane / metabolism
  • Molecular Sequence Data

Substances

  • Biofuels
  • Methane

Associated data

  • GENBANK/HE804839
  • GENBANK/HE804840
  • GENBANK/HE804841
  • GENBANK/HE804842
  • GENBANK/HE804843
  • GENBANK/HE804844
  • GENBANK/HE804845
  • GENBANK/HE804846
  • GENBANK/HE804847
  • GENBANK/HE804848
  • GENBANK/HE804849
  • GENBANK/HE804850
  • GENBANK/HE804851
  • GENBANK/HE804852
  • GENBANK/HE804853
  • GENBANK/HE804854
  • GENBANK/HE804855
  • GENBANK/HE804856
  • GENBANK/HE804857
  • GENBANK/HE804858
  • GENBANK/HE804859
  • GENBANK/HE804860
  • GENBANK/HE804861
  • GENBANK/HE804862
  • GENBANK/HE804863
  • GENBANK/HE804864
  • GENBANK/HE804865
  • GENBANK/HE804866
  • GENBANK/HE804867
  • GENBANK/HE804868
  • GENBANK/HE804869
  • GENBANK/HE804870
  • GENBANK/HE804871
  • GENBANK/HE804872
  • GENBANK/HE804873
  • GENBANK/HE804874
  • GENBANK/HE804875
  • GENBANK/HE804876
  • GENBANK/HE804877
  • GENBANK/HE804878
  • GENBANK/HE804879
  • GENBANK/HE804880
  • GENBANK/HE804881
  • GENBANK/HE804882
  • GENBANK/HE804883
  • GENBANK/HE804884
  • GENBANK/HE804885
  • GENBANK/HE804886
  • GENBANK/HE804887
  • GENBANK/HE804888
  • GENBANK/HE804889
  • GENBANK/HE804890
  • GENBANK/HE804891
  • GENBANK/HE804892
  • GENBANK/HE804893
  • GENBANK/HE804894
  • GENBANK/HE804895
  • GENBANK/HE804896
  • GENBANK/HE804897
  • GENBANK/HE804898
  • GENBANK/HE804899
  • GENBANK/HE804900
  • GENBANK/HE804901
  • GENBANK/HE804902
  • GENBANK/HE804903
  • GENBANK/HE804904
  • GENBANK/HE804905
  • GENBANK/HE804906
  • GENBANK/HE804907
  • GENBANK/HE804908
  • GENBANK/HE804909
  • GENBANK/HE804910
  • GENBANK/HE804911
  • GENBANK/HE804912
  • GENBANK/HE804913
  • GENBANK/HE804914
  • GENBANK/HE804915
  • GENBANK/HE804916
  • GENBANK/HE804917
  • GENBANK/HE804918
  • GENBANK/HE804919
  • GENBANK/HE804920
  • GENBANK/HE804921
  • GENBANK/HE804922
  • GENBANK/HE804923
  • GENBANK/HE804924
  • GENBANK/HE804925
  • GENBANK/HE804926
  • GENBANK/HE804927
  • GENBANK/HE804928
  • GENBANK/HE804929
  • GENBANK/HE804930
  • GENBANK/HE804931
  • GENBANK/HE804932
  • GENBANK/HE804933
  • GENBANK/HE804934
  • GENBANK/HE804935
  • GENBANK/HE804936
  • GENBANK/HE804937
  • GENBANK/HE804938
  • GENBANK/HE804939
  • GENBANK/HE804940
  • GENBANK/HE804941
  • GENBANK/HE804942
  • GENBANK/HE804943
  • GENBANK/HE804944
  • GENBANK/HE804945
  • GENBANK/HE804946
  • GENBANK/HE804947
  • GENBANK/HE804948
  • GENBANK/HE804949
  • GENBANK/HE804950
  • GENBANK/HE804951
  • GENBANK/HE804952
  • GENBANK/HE804953
  • GENBANK/HE804954
  • GENBANK/HE804955
  • GENBANK/HE804956
  • GENBANK/HE804957
  • GENBANK/HE804958
  • GENBANK/HE804959
  • GENBANK/HE804960
  • GENBANK/HE804961
  • GENBANK/HE804962
  • GENBANK/HE804963
  • GENBANK/HE804964
  • GENBANK/HE804965
  • GENBANK/HE804966
  • GENBANK/HE804967
  • GENBANK/HE804968
  • GENBANK/HE804969
  • GENBANK/HE804970
  • GENBANK/HE804971
  • GENBANK/HE804972
  • GENBANK/HE804973
  • GENBANK/HE804974
  • GENBANK/HE804975
  • GENBANK/HE804976
  • GENBANK/HE804977
  • GENBANK/HE804978
  • GENBANK/HE804979
  • GENBANK/HE804980
  • GENBANK/HE804981
  • GENBANK/HE804982
  • GENBANK/HE804983
  • GENBANK/HE804984
  • GENBANK/HE804985
  • GENBANK/HE804986
  • GENBANK/HE804987
  • GENBANK/HE804988
  • GENBANK/HE804989
  • GENBANK/HE804990
  • GENBANK/HE804991
  • GENBANK/HE804992
  • GENBANK/HE804993
  • GENBANK/HE804994
  • GENBANK/HE804995
  • GENBANK/HE804996
  • GENBANK/HE804997
  • GENBANK/HE804998
  • GENBANK/HE804999
  • GENBANK/HE805000
  • GENBANK/HE805001
  • GENBANK/HE805002
  • GENBANK/HE805003
  • GENBANK/HE805004
  • GENBANK/HE805005
  • GENBANK/HE805006
  • GENBANK/HE805007
  • GENBANK/HE805008
  • GENBANK/HE805009
  • GENBANK/HE805010
  • GENBANK/HE805011
  • GENBANK/HE805012
  • GENBANK/HE805013
  • GENBANK/HE805014
  • GENBANK/HE805015
  • GENBANK/HE805016
  • GENBANK/HE805017
  • GENBANK/HE805018
  • GENBANK/HE805019
  • GENBANK/HE805020
  • GENBANK/HE805021
  • GENBANK/HE805022
  • GENBANK/HE805023
  • GENBANK/HE805024
  • GENBANK/HE805025
  • GENBANK/HE805026
  • GENBANK/HE805027
  • GENBANK/HE805028
  • GENBANK/HE805029
  • GENBANK/HE805030
  • GENBANK/HE805031
  • GENBANK/HE805032
  • GENBANK/HE805033
  • GENBANK/HE805034
  • GENBANK/HE805035
  • GENBANK/HE805036
  • GENBANK/HE805037
  • GENBANK/HE805038
  • GENBANK/HE805039
  • GENBANK/HE805040
  • GENBANK/HE805041
  • GENBANK/HE805042
  • GENBANK/HE805043
  • GENBANK/HE805044
  • GENBANK/HE805045
  • GENBANK/HE805046
  • GENBANK/HE805047
  • GENBANK/HE805048
  • GENBANK/HE805049
  • GENBANK/HE805050
  • GENBANK/HE805051
  • GENBANK/HE805052
  • GENBANK/HE805053
  • GENBANK/HE805054
  • GENBANK/HE805055
  • GENBANK/HE805056
  • GENBANK/HE805057
  • GENBANK/HE805058
  • GENBANK/HE805059
  • GENBANK/HE805060
  • GENBANK/HE805061
  • GENBANK/HE805062
  • GENBANK/HE805063
  • GENBANK/HE805064
  • GENBANK/HE805065
  • GENBANK/HE805066
  • GENBANK/HE805067
  • GENBANK/HE805068
  • GENBANK/HE805069
  • GENBANK/HE805070
  • GENBANK/HE805071
  • GENBANK/HE805072
  • GENBANK/HE805073
  • GENBANK/HE805074
  • GENBANK/HE805075
  • GENBANK/HE805076