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Page 1
Common buffers, media, and stock solutions.
[No authors listed] [No authors listed] Curr Protoc Hum Genet. 2001 May;Appendix 2:Appendix 2D. doi: 10.1002/0471142905.hga02ds26. Curr Protoc Hum Genet. 2001. PMID: 18428217
This appendix describes the preparation of selected bacterial media and of buffers and reagents used in the manipulation of nucleic acids and proteins. Recipes for cell culture media and reagents are located elsewhere in the manual. RECIPES: Acids, concentrated stock solut …
This appendix describes the preparation of selected bacterial media and of buffers and reagents used in the manipulation of nucleic a …
Neomenthol prevents the proliferation of skin cancer cells by restraining tubulin polymerization and hyaluronidase activity.
Fatima K, Masood N, Ahmad Wani Z, Meena A, Luqman S. Fatima K, et al. J Adv Res. 2021 Jun 10;34:93-107. doi: 10.1016/j.jare.2021.06.003. eCollection 2021 Dec. J Adv Res. 2021. PMID: 35024183 Free PMC article.
Further, the potency of neomenthol was confirmed by quantitative real-time PCR analysis and molecular docking studies. The in vivo anticancer potential of neomenthol was performed on mice EAC model and the toxicity examination was accomplished through in silico, ex vivo an …
Further, the potency of neomenthol was confirmed by quantitative real-time PCR analysis and molecular docking studies. The in vivo an …
Endoplasmic reticulum stress associated with lead (Pb)-induced olfactory epithelium toxicity in an olfactory dark basal cell line.
Han B, Kamogashira T, Kikuta S, Yamasoba T. Han B, et al. FEBS Open Bio. 2023 Dec;13(12):2162-2171. doi: 10.1002/2211-5463.13714. Epub 2023 Nov 20. FEBS Open Bio. 2023. PMID: 37803507 Free PMC article.
Representative markers of ER stress, apoptosis, and necroptosis were analyzed by quantitative PCR. The mRNA expression levels of GRP94, GRP78, spliced XBP1, PERK, and ATF6 increased significantly after Pb exposure in a dose-dependent manner. ...These results indicate that …
Representative markers of ER stress, apoptosis, and necroptosis were analyzed by quantitative PCR. The mRNA expression levels of GRP9 …
HOPE--a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of > 20 kb from paraffin-embedded tissues. Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect.
Wiedorn KH, Olert J, Stacy RA, Goldmann T, Kühl H, Matthus J, Vollmer E, Bosse A. Wiedorn KH, et al. Pathol Res Pract. 2002;198(11):735-40. doi: 10.1078/0344-0338-00329. Pathol Res Pract. 2002. PMID: 12530576
We thus endeavored to design a new fixing system, including subsequent paraffin-embedding and sectioning, that makes complete pathologic analyses possible, with special consideration of immunohistochemistry (IHC), in situ hybridization (ISH), and molecular pathology. The optimize …
We thus endeavored to design a new fixing system, including subsequent paraffin-embedding and sectioning, that makes complete pathologic ana …
Real-time PCR assay for quantitative mismatch detection.
Shively L, Chang L, LeBon JM, Liu Q, Riggs AD, Singer-Sam J. Shively L, et al. Biotechniques. 2003 Mar;34(3):498-502, 504. doi: 10.2144/03343st01. Biotechniques. 2003. PMID: 12669699 Free article.
Following PCR or RT-PCR of a gene segment that may contain allele-specific differences, 100 pg amplified product are used for a real-time PCR with allele-specific primers and SYBR Green. The use of HEPES buffer at a pH of 6.95 together with Ampl …
Following PCR or RT-PCR of a gene segment that may contain allele-specific differences, 100 pg amplified product are used for …
RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues.
Witchell J, Varshney D, Gajjar T, Wangoo A, Goyal M. Witchell J, et al. Pathol Res Pract. 2008;204(2):105-11. doi: 10.1016/j.prp.2007.09.002. Epub 2007 Nov 5. Pathol Res Pract. 2008. PMID: 17981405
The use of ribonucleic acid (RNA) extracted from Hepes glutamic acid buffer-mediated organic solvent protection effect (HOPE)-fixed tissues in quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is fairly novel. ...In conclusion, the HOPE f …
The use of ribonucleic acid (RNA) extracted from Hepes glutamic acid buffer-mediated organic solvent protection effect (HOPE)- …
A simple and sensitive transient ITP method for on-chip analysis of PCR samples.
Wang L, Liu D, Chen H, Zhou X. Wang L, et al. Electrophoresis. 2008 Dec;29(24):4976-83. doi: 10.1002/elps.200800258. Electrophoresis. 2008. PMID: 19130577
The PCR sample was injected into a 4 mm free-solution buffer channel to increase sample loading. ...The TITP method is simple, fast and sensitive, thus is well suited to direct analysis of the highly saline PCR samples....
The PCR sample was injected into a 4 mm free-solution buffer channel to increase sample loading. ...The TITP method is simple, …
Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
Shahidullah M, To CH, Pelis RM, Delamere NA. Shahidullah M, et al. Invest Ophthalmol Vis Sci. 2009 Apr;50(4):1791-800. doi: 10.1167/iovs.08-2487. Epub 2008 Nov 14. Invest Ophthalmol Vis Sci. 2009. PMID: 19011010 Free PMC article.
In cultured NPE superfused with a CO(2)/HCO(3)(-)-free HEPES buffer, exposure to a CO(2)/HCO(3)(-)-containing buffer caused rapid acidification followed by a gradual increase in pH(i). Subsequent removal of CO(2)/HCO(3)(-) with HEPES buffer caus …
In cultured NPE superfused with a CO(2)/HCO(3)(-)-free HEPES buffer, exposure to a CO(2)/HCO(3)(-)-containing buffer ca …
Fructose modulates cardiomyocyte excitation-contraction coupling and Ca²⁺ handling in vitro.
Mellor KM, Bell JR, Wendt IR, Davidoff AJ, Ritchie RH, Delbridge LM. Mellor KM, et al. PLoS One. 2011;6(9):e25204. doi: 10.1371/journal.pone.0025204. Epub 2011 Sep 29. PLoS One. 2011. PMID: 21980397 Free PMC article.
METHODS AND RESULTS: The functional effects of fructose on isolated adult rat cardiomyocyte contractility and Ca2+ handling were evaluated under physiological conditions (37C, 2 mM Ca2+, HEPES buffer, 4 Hz stimulation) using video edge detection and microfluorimetry …
METHODS AND RESULTS: The functional effects of fructose on isolated adult rat cardiomyocyte contractility and Ca2+ handling were evaluated u …
PCR and culture identification of pathogenic Leptospira spp. from coastal soil in Leyte, Philippines, after a storm surge during Super Typhoon Haiyan (Yolanda).
Saito M, Miyahara S, Villanueva SY, Aramaki N, Ikejiri M, Kobayashi Y, Guevarra JP, Masuzawa T, Gloriani NG, Yanagihara Y, Yoshida S. Saito M, et al. Appl Environ Microbiol. 2014 Nov;80(22):6926-32. doi: 10.1128/AEM.02568-14. Epub 2014 Aug 29. Appl Environ Microbiol. 2014. PMID: 25172869 Free PMC article.
We collected 23 wet soil samples along the coastal areas of Tacloban and Palo 2 months after the storm surge. The samples were suspended in HEPES buffer, and the supernatants were cultured in liquid or semisolid Korthof's medium supplemented with five antimicrobial …
We collected 23 wet soil samples along the coastal areas of Tacloban and Palo 2 months after the storm surge. The samples were suspended in …
57 results