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Allosteric mechanisms in P450eryF probed with 1-pyrenebutanol, a novel fluorescent substrate.
Davydov DR, Kumar S, Halpert JR. Davydov DR, et al. Among authors: halpert jr. Biochem Biophys Res Commun. 2002 Jun 21;294(4):806-12. doi: 10.1016/S0006-291X(02)00565-X. Biochem Biophys Res Commun. 2002. PMID: 12061778
Functional role of residues in the helix B' region of cytochrome P450 2B1.
Honma W, Li W, Liu H, Scott EE, Halpert JR. Honma W, et al. Among authors: halpert jr. Arch Biochem Biophys. 2005 Mar 1;435(1):157-65. doi: 10.1016/j.abb.2004.12.014. Arch Biochem Biophys. 2005. PMID: 15680917
Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide.
Kumar S, Chen CS, Waxman DJ, Halpert JR. Kumar S, et al. Among authors: halpert jr. J Biol Chem. 2005 May 20;280(20):19569-75. doi: 10.1074/jbc.M500158200. Epub 2005 Mar 17. J Biol Chem. 2005. PMID: 15774478
Role of cytochrome B5 in modulating peroxide-supported cyp3a4 activity: evidence for a conformational transition and cytochrome P450 heterogeneity.
Kumar S, Davydov DR, Halpert JR. Kumar S, et al. Among authors: halpert jr. Drug Metab Dispos. 2005 Aug;33(8):1131-6. doi: 10.1124/dmd.105.004606. Epub 2005 May 3. Drug Metab Dispos. 2005. PMID: 15870379
Allosteric P450 mechanisms: multiple binding sites, multiple conformers or both?
Davydov DR, Halpert JR. Davydov DR, et al. Among authors: halpert jr. Expert Opin Drug Metab Toxicol. 2008 Dec;4(12):1523-35. doi: 10.1517/17425250802500028. Expert Opin Drug Metab Toxicol. 2008. PMID: 19040328 Free PMC article. Review.
Multiple substrate-binding sites are retained in cytochrome P450 3A4 mutants with decreased cooperativity.
Fernando H, Rumfeldt JA, Davydova NY, Halpert JR, Davydov DR. Fernando H, et al. Among authors: halpert jr. Xenobiotica. 2011 Apr;41(4):281-9. doi: 10.3109/00498254.2010.538748. Epub 2010 Dec 14. Xenobiotica. 2011. PMID: 21143007 Free PMC article.
Thermodynamic fidelity of the mammalian cytochrome P450 2B4 active site in binding substrates and inhibitors.
Muralidhara BK, Sun L, Negi S, Halpert JR. Muralidhara BK, et al. Among authors: halpert jr. J Mol Biol. 2008 Mar 14;377(1):232-45. doi: 10.1016/j.jmb.2007.12.068. Epub 2008 Jan 5. J Mol Biol. 2008. PMID: 18241887
Effect of glutathione on homo- and heterotropic cooperativity in cytochrome P450 3A4.
Davydov DR, Davydova NY, Tsalkova TN, Halpert JR. Davydov DR, et al. Among authors: halpert jr. Arch Biochem Biophys. 2008 Mar 15;471(2):134-45. doi: 10.1016/j.abb.2008.01.001. Epub 2008 Jan 11. Arch Biochem Biophys. 2008. PMID: 18206979 Free PMC article.
Kinetics of electron transfer in the complex of cytochrome P450 3A4 with the flavin domain of cytochrome P450BM-3 as evidence of functional heterogeneity of the heme protein.
Fernando H, Halpert JR, Davydov DR. Fernando H, et al. Among authors: halpert jr. Arch Biochem Biophys. 2008 Mar 1;471(1):20-31. doi: 10.1016/j.abb.2007.11.020. Epub 2007 Dec 7. Arch Biochem Biophys. 2008. PMID: 18086551 Free PMC article.
Structural and thermodynamic consequences of 1-(4-chlorophenyl)imidazole binding to cytochrome P450 2B4.
Zhao Y, Sun L, Muralidhara BK, Kumar S, White MA, Stout CD, Halpert JR. Zhao Y, et al. Among authors: halpert jr. Biochemistry. 2007 Oct 16;46(41):11559-67. doi: 10.1021/bi7011614. Epub 2007 Sep 22. Biochemistry. 2007. PMID: 17887776 Free PMC article.
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