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Expression of the Pseudomonas aeruginosa toxA positive regulatory gene (regA) in Escherichia coli.
Hamood AN, Iglewski BH. Hamood AN, et al. J Bacteriol. 1990 Feb;172(2):589-94. doi: 10.1128/jb.172.2.589-594.1990. J Bacteriol. 1990. PMID: 2105298 Free PMC article.
Production of the RegA protein in E. coli required both an E. coli promoter and an E. coli ribosome-binding site. Two in-frame deletion derivatives in which certain regions of the regA gene were expressed from the T7 promoter encoded 26- and 18-kilodalton fusion pro …
Production of the RegA protein in E. coli required both an E. coli promoter and an E. coli ribosome-binding site. Two in-frame …
Secretion of toxin A from Pseudomonas aeruginosa PAO1, PAK, and PA103 by Escherichia coli.
Hamood AN, Wick MJ, Iglewski BH. Hamood AN, et al. Infect Immun. 1990 May;58(5):1133-40. doi: 10.1128/IAI.58.5.1133-1140.1990. Infect Immun. 1990. PMID: 2108926 Free PMC article.
This suggests the presence of an E. coli periplasmic factor that interferes with the ADP-ribosyltransferase activity in toxin A. Such an inactivating factor was found in the periplasmic extract from control E. coli cells. The processing of toxin A in E. coli was exa …
This suggests the presence of an E. coli periplasmic factor that interferes with the ADP-ribosyltransferase activity in toxin A. Such …
Analysis of the structure-function relationship of Pseudomonas aeruginosa exotoxin A.
Wick MJ, Hamood AN, Iglewski BH. Wick MJ, et al. Mol Microbiol. 1990 Apr;4(4):527-35. doi: 10.1111/j.1365-2958.1990.tb00620.x. Mol Microbiol. 1990. PMID: 2112672 Review.
Several residues within domain III have been identified as having a direct role in catalysis, while others are hypothesized to play an important structural role....
Several residues within domain III have been identified as having a direct role in catalysis, while others are hypothesized to play an
Regions of toxin A involved in toxin A excretion in Pseudomonas aeruginosa.
Hamood AN, Olson JC, Vincent TS, Iglewski BH. Hamood AN, et al. J Bacteriol. 1989 Apr;171(4):1817-24. doi: 10.1128/jb.171.4.1817-1824.1989. J Bacteriol. 1989. PMID: 2495264 Free PMC article.
The data suggest that the amino terminus region of toxin A (the leader peptide plus the first 30 amino acid of the mature protein) is sufficient for its excretion, and that a second region, amino acids 309 through 413, protects an internally truncated toxin A molecule from …
The data suggest that the amino terminus region of toxin A (the leader peptide plus the first 30 amino acid of the mature protein) is suffic …
Characterization of a gene that regulates toxin A synthesis in Pseudomonas aeruginosa.
Hindahl MS, Frank DW, Hamood A, Iglewski BH. Hindahl MS, et al. Nucleic Acids Res. 1988 Jun 24;16(12):5699. doi: 10.1093/nar/16.12.5699. Nucleic Acids Res. 1988. PMID: 3133641 Free PMC article. No abstract available.
Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A.
West SE, Kaye SA, Hamood AN, Iglewski BH. West SE, et al. Infect Immun. 1994 Mar;62(3):897-903. doi: 10.1128/IAI.62.3.897-903.1994. Infect Immun. 1994. PMID: 8112861 Free PMC article.
In Pseudomonas aeruginosa, production of exotoxin A, an ADP-ribosyltransferase, is a complex and highly regulated process. Two positively acting regulatory genes, regA and regB, have been cloned and characterized. ...This observation indicates that the lesion(s) in the mut …
In Pseudomonas aeruginosa, production of exotoxin A, an ADP-ribosyltransferase, is a complex and highly regulated process. Two positi …
DNA hybridization analysis of the Pseudomonas aeruginosa elastase gene (lasB) from different clinical isolates.
Hamood AN, Griswold J. Hamood AN, et al. Can J Microbiol. 1995 Oct;41(10):910-7. doi: 10.1139/m95-125. Can J Microbiol. 1995. PMID: 8590405
In that isolate, an additional hybridization band was detected. Immunoblotting experiments confirmed that elastase protein is not produced by 6 out of 67 isolates. ...
In that isolate, an additional hybridization band was detected. Immunoblotting experiments confirmed that elastase protein is not pro …
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