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The human skin barrier is organized as stacked bilayers of fully extended ceramides with cholesterol molecules associated with the ceramide sphingoid moiety.
Iwai I, Han H, den Hollander L, Svensson S, Ofverstedt LG, Anwar J, Brewer J, Bloksgaard M, Laloeuf A, Nosek D, Masich S, Bagatolli LA, Skoglund U, Norlén L. Iwai I, et al. Among authors: han h. J Invest Dermatol. 2012 Sep;132(9):2215-25. doi: 10.1038/jid.2012.43. Epub 2012 Apr 26. J Invest Dermatol. 2012. PMID: 22534876 Free article.
Cryo-fixation by self-pressurized rapid freezing.
Grabenbauer M, Han HM, Huebinger J. Grabenbauer M, et al. Methods Mol Biol. 2014;1117:173-91. doi: 10.1007/978-1-62703-776-1_9. Methods Mol Biol. 2014. PMID: 24357364
Golgi apparatus analyzed by cryo-electron microscopy.
Han HM, Bouchet-Marquis C, Huebinger J, Grabenbauer M. Han HM, et al. Histochem Cell Biol. 2013 Oct;140(4):369-81. doi: 10.1007/s00418-013-1136-3. Epub 2013 Aug 18. Histochem Cell Biol. 2013. PMID: 23954988 Free PMC article. Review.
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