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Genome engineering using CRISPR-Cas9 system.
Cong L, Zhang F. Cong L, et al. Methods Mol Biol. 2015;1239:197-217. doi: 10.1007/978-1-4939-1862-1_10. Methods Mol Biol. 2015. PMID: 25408407
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that exists in a variety of microbes. ...In this chapter, detailed protocols are described for harnessing the CRISPR-Cas9 syst …
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that ex …
Harnessing CRISPR-Cas9 immunity for genetic engineering.
Charpentier E, Marraffini LA. Charpentier E, et al. Curr Opin Microbiol. 2014 Jun;19:114-119. doi: 10.1016/j.mib.2014.07.001. Epub 2014 Jul 19. Curr Opin Microbiol. 2014. PMID: 25048165 Free PMC article.
CRISPR-Cas encodes an adaptive immune system that defends prokaryotes against infectious viruses and plasmids. Immunity is mediated by Cas nucleases, which use small RNA guides (the crRNAs) to specify a cleavage site within the genome of invading nucleic acid
CRISPR-Cas encodes an adaptive immune system that defends prokaryotes against infectious viruses and plasmids. Immunity
Antiviral Goes Viral: Harnessing CRISPR/Cas9 to Combat Viruses in Humans.
Soppe JA, Lebbink RJ. Soppe JA, et al. Trends Microbiol. 2017 Oct;25(10):833-850. doi: 10.1016/j.tim.2017.04.005. Epub 2017 May 15. Trends Microbiol. 2017. PMID: 28522157 Review.
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are RNA-guided sequence-specific prokaryotic antiviral immune systems. ...A rewired CRISPR/Cas9 system can be used for targeted and precis …
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are RNA-guided sequ …
Engineering Plants for Geminivirus Resistance with CRISPR/Cas9 System.
Zaidi SS, Mansoor S, Ali Z, Tashkandi M, Mahfouz MM. Zaidi SS, et al. Trends Plant Sci. 2016 Apr;21(4):279-281. doi: 10.1016/j.tplants.2016.01.023. Epub 2016 Feb 12. Trends Plant Sci. 2016. PMID: 26880316
The CRISPR/Cas9 system is an efficient genome-editing platform for diverse eukaryotic species, including plants. Recent work harnessed CRISPR/Cas9 technology to engineer resistance to geminiviruses. ...
The CRISPR/Cas9 system is an efficient genome-editing platform for diverse eukaryotic species, including plants. Recent work …
A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants.
Ali Z, Zaidi SS, Tashkandi M, Mahfouz MM. Ali Z, et al. Methods Mol Biol. 2019;2028:167-183. doi: 10.1007/978-1-4939-9635-3_10. Methods Mol Biol. 2019. PMID: 31228115
CRISPR/Cas9 is an adaptive immunity mechanism used by prokaryotes to defend against invading nucleic acids of phages and plasmids. The CRISPR/Cas9 system has been harnessed for targeted genome editing in a variety of eukaryotic species, a
CRISPR/Cas9 is an adaptive immunity mechanism used by prokaryotes to defend against invading nucleic acids of phages an
CRISPR-Cas13d mediates robust RNA virus interference in plants.
Mahas A, Aman R, Mahfouz M. Mahas A, et al. Genome Biol. 2019 Dec 2;20(1):263. doi: 10.1186/s13059-019-1881-2. Genome Biol. 2019. PMID: 31791381 Free PMC article.
BACKGROUND: CRISPR-Cas systems endow bacterial and archaeal species with adaptive immunity mechanisms to fend off invading phages and foreign genetic elements. CRISPR-Cas9 has been harnessed to confer virus interference against DNA viruse …
BACKGROUND: CRISPR-Cas systems endow bacterial and archaeal species with adaptive immunity mechanisms to fend off invading pha …
Unraveling the potential of CRISPR-Cas9 for gene therapy.
Barrangou R, May AP. Barrangou R, et al. Expert Opin Biol Ther. 2015 Mar;15(3):311-4. doi: 10.1517/14712598.2015.994501. Epub 2014 Dec 23. Expert Opin Biol Ther. 2015. PMID: 25535790
The molecular machinery from the prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune system has broadly been repurposed for genome editing in eukaryotes. In particular, the sequence-specific Cas9 endonuclease can be flexi …
The molecular machinery from the prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune sys …
CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering.
Singh A, Chakraborty D, Maiti S. Singh A, et al. Chem Soc Rev. 2016 Dec 21;45(24):6666-6684. doi: 10.1039/c6cs00197a. Epub 2016 Sep 14. Chem Soc Rev. 2016. PMID: 27711765 Review.
The CRISPR-Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplistic model of RNA guided DNA binding and cleavage, this molecular toolbox has found application in nearly every branch of biological sciences …
The CRISPR-Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplis …
Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease.
Limanskiy V, Vyas A, Chaturvedi LS, Vyas D. Limanskiy V, et al. World J Gastroenterol. 2019 May 14;25(18):2177-2187. doi: 10.3748/wjg.v25.i18.2177. World J Gastroenterol. 2019. PMID: 31143069 Free PMC article. Review.
The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease (IBD) and consequently decrease th …
The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas
Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production.
Zhang J, Zong W, Hong W, Zhang ZT, Wang Y. Zhang J, et al. Metab Eng. 2018 May;47:49-59. doi: 10.1016/j.ymben.2018.03.007. Epub 2018 Mar 9. Metab Eng. 2018. PMID: 29530750
Although CRISPR-Cas9/Cpf1 have been employed as powerful genome engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into bacteria and archaea due to their severe toxicity. Since most prokaryotes harbor native CRI
Although CRISPR-Cas9/Cpf1 have been employed as powerful genome engineering tools, heterologous CRISPR-Cas9
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