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Tissue plasminogen activator has an O-linked fucose attached to threonine-61 in the epidermal growth factor domain.
Harris RJ, Leonard CK, Guzzetta AW, Spellman MW. Harris RJ, et al. Biochemistry. 1991 Mar 5;30(9):2311-4. doi: 10.1021/bi00223a004. Biochemistry. 1991. PMID: 1900431
Establishing a control system using QbD principles.
Kepert JF, Cromwell M, Engler N, Finkler C, Gellermann G, Gennaro L, Harris R, Iverson R, Kelley B, Krummen L, McKnight N, Motchnik P, Schnaible V, Taticek R. Kepert JF, et al. Biologicals. 2016 Sep;44(5):319-31. doi: 10.1016/j.biologicals.2016.06.003. Epub 2016 Jul 16. Biologicals. 2016. PMID: 27430904 Review.
Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed.
Chaderjian WB, Chin ET, Harris RJ, Etcheverry TM. Chaderjian WB, et al. Among authors: harris rj. Biotechnol Prog. 2005 Mar-Apr;21(2):550-3. doi: 10.1021/bp0497029. Biotechnol Prog. 2005. PMID: 15801797
Non-enzymatic hinge region fragmentation of antibodies in solution.
Cordoba AJ, Shyong BJ, Breen D, Harris RJ. Cordoba AJ, et al. Among authors: harris rj. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Apr 25;818(2):115-21. doi: 10.1016/j.jchromb.2004.12.033. J Chromatogr B Analyt Technol Biomed Life Sci. 2005. PMID: 15734150
Characterization of oligosaccharides in recombinant tissue plasminogen activator produced in Chinese hamster ovary cells: two decades of analytical technology development.
Borisov OV, Field M, Ling VT, Harris RJ. Borisov OV, et al. Among authors: harris rj. Anal Chem. 2009 Dec 1;81(23):9744-54. doi: 10.1021/ac901498k. Anal Chem. 2009. PMID: 19947664
A sensitive mutation screening method supporting cell line development for biotherapeutics.
Valisheva I, Harris RJ, Zhu-Shimoni J. Valisheva I, et al. Among authors: harris rj. Anal Biochem. 2016 Jul 15;505:73-5. doi: 10.1016/j.ab.2016.04.007. Epub 2016 Apr 20. Anal Biochem. 2016. PMID: 27108188
Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase.
Wang Y, Shao L, Shi S, Harris RJ, Spellman MW, Stanley P, Haltiwanger RS. Wang Y, et al. Among authors: harris rj. J Biol Chem. 2001 Oct 26;276(43):40338-45. doi: 10.1074/jbc.M107849200. Epub 2001 Aug 27. J Biol Chem. 2001. PMID: 11524432
Identification of multiple sources of charge heterogeneity in a recombinant antibody.
Harris RJ, Kabakoff B, Macchi FD, Shen FJ, Kwong M, Andya JD, Shire SJ, Bjork N, Totpal K, Chen AB. Harris RJ, et al. J Chromatogr B Biomed Sci Appl. 2001 Mar 10;752(2):233-45. doi: 10.1016/s0378-4347(00)00548-x. J Chromatogr B Biomed Sci Appl. 2001. PMID: 11270864
Hydroxylation of Lys residues reduces their susceptibility to digestion by trypsin and lysyl endopeptidase.
Molony MS, Quan C, Mulkerrin MG, Harris RJ. Molony MS, et al. Among authors: harris rj. Anal Biochem. 1998 Apr 10;258(1):136-7. doi: 10.1006/abio.1997.2513. Anal Biochem. 1998. PMID: 9527859 No abstract available.
O-linked fucose and other post-translational modifications unique to EGF modules.
Harris RJ, Spellman MW. Harris RJ, et al. Glycobiology. 1993 Jun;3(3):219-24. doi: 10.1093/glycob/3.3.219. Glycobiology. 1993. PMID: 8358148 Review.
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