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Rapid and quantitative detection of human adenovirus DNA by real-time PCR.
Heim A, Ebnet C, Harste G, Pring-Akerblom P. Heim A, et al. J Med Virol. 2003 Jun;70(2):228-39. doi: 10.1002/jmv.10382. J Med Virol. 2003. PMID: 12696109
Therefore, a quantitative "real-time" PCR with consensus primer and probe (dual fluorescence labelled, "TaqMan") sequences for a conserved region of the hexon gene was designed and evaluated. ...In conclusion, real-time PCR is a sensitive and quantitative pro …
Therefore, a quantitative "real-time" PCR with consensus primer and probe (dual fluorescence labelled, "TaqMan") sequences for a
Conserved sequences in the fibers of epidemic keratoconjunctivitis associated human adenoviruses.
Pring-Akerblom P, Heim A, Trijssenaar FE. Pring-Akerblom P, et al. Arch Virol. 1997;142(1):205-11. doi: 10.1007/s007050050071. Arch Virol. 1997. PMID: 9155885
The predicted polypeptides exposed a high homology. Ad8, Ad19, and Ad37 can cause epidemic keratoconjunctivitis (EKC), whereas Ad9 only infrequently causes acute follicular conjunctivitis. ...
The predicted polypeptides exposed a high homology. Ad8, Ad19, and Ad37 can cause epidemic keratoconjunctivitis (EKC), whereas Ad9 on …
Enterovirus heart disease of adults: a persistent, limited organ infection in the presence of neutralizing antibodies.
Heim A, Grumbach I, Hake S, Müller G, Pring-Akerblom P, Mall G, Figulla HR. Heim A, et al. J Med Virol. 1997 Nov;53(3):196-204. doi: 10.1002/(sici)1096-9071(199711)53:3<196::aid-jmv3>3.0.co;2-3. J Med Virol. 1997. PMID: 9365882
In addition, enterovirus RNA was demonstrated in PBMC, but not in EMB, of a single patient with suspected EHD. A high sequence homology of the amplicons to coxsackievirus B3 was demonstrated in 7 patients, and to coxsackievirus B2 in two patients. ...
In addition, enterovirus RNA was demonstrated in PBMC, but not in EMB, of a single patient with suspected EHD. A high sequence …
Antiviral activity of WIN 54954 in coxsackievirus B2 carrier state infected human myocardial fibroblasts.
Heim A, Pfetzing U, Müller G, Grumbach IM. Heim A, et al. Antiviral Res. 1998 Jan;37(1):47-56. doi: 10.1016/s0166-3542(97)00056-9. Antiviral Res. 1998. PMID: 9497072
Persistent infections with a cardiotropic enterovirus, e.g. coxsackievirus B2 (CVB2), cause chronic myocarditis and eventually congestive heart failure. ...In conclusion, the results indicate that low concentrations of WIN 54954 are effective in treating persistent enterov …
Persistent infections with a cardiotropic enterovirus, e.g. coxsackievirus B2 (CVB2), cause chronic myocarditis and eventually conges …
Highly sensitive detection of gene expression of an intronless gene: amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA).
Heim A, Grumbach IM, Zeuke S, Top B. Heim A, et al. Nucleic Acids Res. 1998 May 1;26(9):2250-1. doi: 10.1093/nar/26.9.2250. Nucleic Acids Res. 1998. PMID: 9547289 Free PMC article.
NASBA is an isothermal nucleic acid amplification reaction that amplifies mRNA in a dsDNA background. Although similar to the sensitive reverse transcription/polymerase chain reaction (RT-PCR) in mRNA detection, NASBA is not prone to give false positive results caused by g …
NASBA is an isothermal nucleic acid amplification reaction that amplifies mRNA in a dsDNA background. Although similar to the sensiti …
Evaluation of serological screening of cadaveric sera for donor selection for cornea transplantation.
Heim A, Wagner D, Rothämel T, Hartmann U, Flik J, Verhagen W. Heim A, et al. J Med Virol. 1999 Jul;58(3):291-5. doi: 10.1002/(sici)1096-9071(199907)58:3<291::aid-jmv16>3.0.co;2-d. J Med Virol. 1999. PMID: 10447426
Virus safety of the graft was affected in a single case, which was HCV antibody negative in the cadaveric serum, but positive in the premortem serum (confirmed by HCV-RIBA strip immunoassay). ...In conclusion, the results indicate that serological testing of cadaveric sera …
Virus safety of the graft was affected in a single case, which was HCV antibody negative in the cadaveric serum, but positive in the …
Transient induction of cytokine production in human myocardial fibroblasts by coxsackievirus B3.
Heim A, Zeuke S, Weiss S, Ruschewski W, Grumbach IM. Heim A, et al. Circ Res. 2000 Apr 14;86(7):753-9. doi: 10.1161/01.res.86.7.753. Circ Res. 2000. PMID: 10764408
As early as 3 hours after infection, RT-qPCR demonstrated a 2-fold increase of interleukin (IL)-6 and IL-8 mRNA compared with basal transcription, resulting in a significant increase of IL-6 and IL-8 to a median level of 1500 pg/mL (range, 1246 to 1858) and 5 …
As early as 3 hours after infection, RT-qPCR demonstrated a 2-fold increase of interleukin (IL)-6 and IL-8 mRNA compared with basal t …
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