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Strong cation exchange-based fractionation of Lys-N-generated peptides facilitates the targeted analysis of post-translational modifications.
Taouatas N, Altelaar AF, Drugan MM, Helbig AO, Mohammed S, Heck AJ. Taouatas N, et al. Mol Cell Proteomics. 2009 Jan;8(1):190-200. doi: 10.1074/mcp.M800285-MCP200. Epub 2008 Sep 29. Mol Cell Proteomics. 2009. PMID: 18824475
The diversity of protein turnover and abundance under nitrogen-limited steady-state conditions in Saccharomyces cerevisiae.
Helbig AO, Daran-Lapujade P, van Maris AJ, de Hulster EA, de Ridder D, Pronk JT, Heck AJ, Slijper M. Helbig AO, et al. Mol Biosyst. 2011 Dec;7(12):3316-26. doi: 10.1039/c1mb05250k. Epub 2011 Oct 10. Mol Biosyst. 2011. PMID: 21984188
Perturbation of the yeast N-acetyltransferase NatB induces elevation of protein phosphorylation levels.
Helbig AO, Rosati S, Pijnappel PW, van Breukelen B, Timmers MH, Mohammed S, Slijper M, Heck AJ. Helbig AO, et al. BMC Genomics. 2010 Dec 2;11:685. doi: 10.1186/1471-2164-11-685. BMC Genomics. 2010. PMID: 21126336 Free PMC article.
Exploring the membrane proteome--challenges and analytical strategies.
Helbig AO, Heck AJ, Slijper M. Helbig AO, et al. J Proteomics. 2010 Mar 10;73(5):868-78. doi: 10.1016/j.jprot.2010.01.005. Epub 2010 Jan 21. J Proteomics. 2010. PMID: 20096812 Review.
Profiling of N-acetylated protein termini provides in-depth insights into the N-terminal nature of the proteome.
Helbig AO, Gauci S, Raijmakers R, van Breukelen B, Slijper M, Mohammed S, Heck AJ. Helbig AO, et al. Mol Cell Proteomics. 2010 May;9(5):928-39. doi: 10.1074/mcp.M900463-MCP200. Epub 2010 Jan 7. Mol Cell Proteomics. 2010. PMID: 20061308 Free PMC article.
A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions.
Helbig AO, de Groot MJ, van Gestel RA, Mohammed S, de Hulster EA, Luttik MA, Daran-Lapujade P, Pronk JT, Heck AJ, Slijper M. Helbig AO, et al. Proteomics. 2009 Oct;9(20):4787-98. doi: 10.1002/pmic.200800951. Proteomics. 2009. PMID: 19750512
Combination of SCX Fractionation and Charge-Reversal Derivatization Facilitates the Identification of Nontryptic Peptides in C-Terminomics.
Kaleja P, Helbig AO, Tholey A. Kaleja P, et al. Among authors: helbig ao. J Proteome Res. 2019 Jul 5;18(7):2954-2964. doi: 10.1021/acs.jproteome.9b00264. Epub 2019 Jun 25. J Proteome Res. 2019. PMID: 31195796
Degradome of soluble ADAM10 and ADAM17 metalloproteases.
Scharfenberg F, Helbig A, Sammel M, Benzel J, Schlomann U, Peters F, Wichert R, Bettendorff M, Schmidt-Arras D, Rose-John S, Moali C, Lichtenthaler SF, Pietrzik CU, Bartsch JW, Tholey A, Becker-Pauly C. Scharfenberg F, et al. Cell Mol Life Sci. 2020 Jan;77(2):331-350. doi: 10.1007/s00018-019-03184-4. Epub 2019 Jun 17. Cell Mol Life Sci. 2020. PMID: 31209506
Atherogenic LOX-1 signaling is controlled by SPPL2-mediated intramembrane proteolysis.
Mentrup T, Theodorou K, Cabrera-Cabrera F, Helbig AO, Happ K, Gijbels M, Gradtke AC, Rabe B, Fukumori A, Steiner H, Tholey A, Fluhrer R, Donners M, Schröder B. Mentrup T, et al. Among authors: helbig ao. J Exp Med. 2019 Apr 1;216(4):807-830. doi: 10.1084/jem.20171438. Epub 2019 Feb 28. J Exp Med. 2019. PMID: 30819724 Free PMC article.
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