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Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells.
Hemeda H, Giebel B, Wagner W. Hemeda H, et al. Cytotherapy. 2014 Feb;16(2):170-80. doi: 10.1016/j.jcyt.2013.11.004. Cytotherapy. 2014. PMID: 24438898
Human nasal mucosa contains tissue-resident immunologically responsive mesenchymal stromal cells.
Jakob M, Hemeda H, Janeschik S, Bootz F, Rotter N, Lang S, Brandau S. Jakob M, et al. Among authors: hemeda h. Stem Cells Dev. 2010 May;19(5):635-44. doi: 10.1089/scd.2009.0245. Stem Cells Dev. 2010. PMID: 19761404
Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells.
Hemeda H, Jakob M, Ludwig AK, Giebel B, Lang S, Brandau S. Hemeda H, et al. Stem Cells Dev. 2010 May;19(5):693-706. doi: 10.1089/scd.2009.0365. Stem Cells Dev. 2010. PMID: 20067407
Tissue-resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge.
Brandau S, Jakob M, Hemeda H, Bruderek K, Janeschik S, Bootz F, Lang S. Brandau S, et al. Among authors: hemeda h. J Leukoc Biol. 2010 Nov;88(5):1005-15. doi: 10.1189/jlb.0410207. Epub 2010 Aug 3. J Leukoc Biol. 2010. PMID: 20682625
Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells.
Lohmann M, Walenda G, Hemeda H, Joussen S, Drescher W, Jockenhoevel S, Hutschenreuter G, Zenke M, Wagner W. Lohmann M, et al. Among authors: hemeda h. PLoS One. 2012;7(5):e37839. doi: 10.1371/journal.pone.0037839. Epub 2012 May 25. PLoS One. 2012. PMID: 22662236 Free PMC article.
Human platelet lysate gel provides a novel three dimensional-matrix for enhanced culture expansion of mesenchymal stromal cells.
Walenda G, Hemeda H, Schneider RK, Merkel R, Hoffmann B, Wagner W. Walenda G, et al. Among authors: hemeda h. Tissue Eng Part C Methods. 2012 Dec;18(12):924-34. doi: 10.1089/ten.TEC.2011.0541. Epub 2012 Jul 16. Tissue Eng Part C Methods. 2012. PMID: 22670863
Heparin needs to be added as anticoagulant before addition of HPL to culture medium; otherwise, HPL-medium forms a gel within 1 h. ...
Heparin needs to be added as anticoagulant before addition of HPL to culture medium; otherwise, HPL-medium forms a gel within 1 h. .. …
Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: potential impact on wound healing, trauma, and infection.
Jakob M, Hemeda H, Bruderek K, H Gerstner AO, Bootz F, Lang S, Brandau S. Jakob M, et al. Among authors: hemeda h, h gerstner ao. Head Neck. 2013 Nov;35(11):1621-9. doi: 10.1002/hed.23196. Epub 2012 Nov 14. Head Neck. 2013. PMID: 23152157
Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency.
Schellenberg A, Hemeda H, Wagner W. Schellenberg A, et al. Among authors: hemeda h. Methods Mol Biol. 2013;976:143-54. doi: 10.1007/978-1-62703-317-6_11. Methods Mol Biol. 2013. PMID: 23400440
To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture.
Willmann CA, Hemeda H, Pieper LA, Lenz M, Qin J, Joussen S, Sontag S, Wanek P, Denecke B, Schüler HM, Zenke M, Wagner W. Willmann CA, et al. Among authors: hemeda h. PLoS One. 2013 May 29;8(5):e65324. doi: 10.1371/journal.pone.0065324. Print 2013. PLoS One. 2013. PMID: 23734247 Free PMC article.
Heparin concentration is critical for cell culture with human platelet lysate.
Hemeda H, Kalz J, Walenda G, Lohmann M, Wagner W. Hemeda H, et al. Cytotherapy. 2013 Sep;15(9):1174-81. doi: 10.1016/j.jcyt.2013.05.006. Epub 2013 Jul 9. Cytotherapy. 2013. PMID: 23845186
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