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High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.
D'Antonio M, Woodruff G, Nathanson JL, D'Antonio-Chronowska A, Arias A, Matsui H, Williams R, Herrera C, Reyna SM, Yeo GW, Goldstein LSB, Panopoulos AD, Frazer KA. D'Antonio M, et al. Stem Cell Reports. 2017 Apr 11;8(4):1101-1111. doi: 10.1016/j.stemcr.2017.03.011. Stem Cell Reports. 2017. PMID: 28410643 Free PMC article.
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocyt …
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying …
Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders.
Zink A, Lisowski P, Prigione A. Zink A, et al. Bio Protoc. 2021 Mar 5;11(5):e3939. doi: 10.21769/BioProtoc.3939. eCollection 2021 Mar 5. Bio Protoc. 2021. PMID: 33796613 Free PMC article.
However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery …
However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human …
Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization.
Oh JG, Dave J, Kho C, Stillitano F. Oh JG, et al. J Vis Exp. 2020 Jan 17;(155). doi: 10.3791/60135. J Vis Exp. 2020. PMID: 32009647
Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) provide a valuable human source for studying the basic science of calcium (Ca(2+)) handling and signaling pathways as well as high-throughput drug screening and toxicity assay …
Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) provide a valuable human source for studying the …
Integration of Engineered "Spark-Cell" Spheroids for Optical Pacing of Cardiac Tissue.
Chua CJ, Han JL, Li W, Liu W, Entcheva E. Chua CJ, et al. Front Bioeng Biotechnol. 2021 Jun 18;9:658594. doi: 10.3389/fbioe.2021.658594. eCollection 2021. Front Bioeng Biotechnol. 2021. PMID: 34222210 Free PMC article.
Optogenetic methods for pacing of cardiac tissue can be realized by direct genetic modification of the cardiomyocytes to express light-sensitive actuators, such as channelrhodopsin-2, ChR2, or by introduction of light-sensitized non-myocytes that couple to the cardiac cells …
Optogenetic methods for pacing of cardiac tissue can be realized by direct genetic modification of the cardiomyocytes to express light-sensi …
Induced cancer stem-like cells as a model for biological screening and discovery of agents targeting phenotypic traits of cancer stem cell.
Nishi M, Akutsu H, Kudoh A, Kimura H, Yamamoto N, Umezawa A, Lee SW, Ryo A. Nishi M, et al. Oncotarget. 2014 Sep 30;5(18):8665-80. doi: 10.18632/oncotarget.2356. Oncotarget. 2014. PMID: 25228591 Free PMC article.
Novel drugs that target CSCs can potentially eliminate the tumor initiating cell population therefore resulting in complete cure of the cancer. We recently established a CSC-like model using induced pluripotent stem cell (iPSC) technology to reprogram and par …
Novel drugs that target CSCs can potentially eliminate the tumor initiating cell population therefore resulting in complete cure of the canc …
Fast Optical Investigation of Cardiac Electrophysiology by Parallel Detection in Multiwell Plates.
Credi C, Balducci V, Munagala U, Cianca C, Bigiarini S, de Vries AAF, Loew LM, Pavone FS, Cerbai E, Sartiani L, Sacconi L. Credi C, et al. Front Physiol. 2021 Sep 3;12:692496. doi: 10.3389/fphys.2021.692496. eCollection 2021. Front Physiol. 2021. PMID: 34539428 Free PMC article.
Here, we describe an alternative cost-effective optical approach (MULTIPLE) that exploits high-power LED arrays to globally illuminate a culture plate and an sCMOS sensor for parallel detection of the fluorescence coming from multiple wells. ...Finally, actio …
Here, we describe an alternative cost-effective optical approach (MULTIPLE) that exploits high-power LED arrays to glob …