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Simultaneous amplification and detection of specific DNA sequences.
Higuchi R, Dollinger G, Walsh PS, Griffith R. Higuchi R, et al. Biotechnology (N Y). 1992 Apr;10(4):413-7. doi: 10.1038/nbt0492-413. Biotechnology (N Y). 1992. PMID: 1368485
Homogeneous allele-specific PCR in SNP genotyping.
Germer S, Higuchi R. Germer S, et al. Among authors: higuchi r. Methods Mol Biol. 2003;212:197-214. doi: 10.1385/1-59259-327-5:197. Methods Mol Biol. 2003. PMID: 12491912 No abstract available.
High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.
Wang J, Chuang K, Ahluwalia M, Patel S, Umblas N, Mirel D, Higuchi R, Germer S. Wang J, et al. Among authors: higuchi r. Biotechniques. 2005 Dec;39(6):885-93. doi: 10.2144/000112028. Biotechniques. 2005. PMID: 16382908
A family with haemolytic anaemia and three beta-globins: the deletion in haemoglobin Atlanta-Coventry (beta 75 Leu----Pro, 141 Leu deleted) is not present at the nucleotide level.
George PM, Myles T, Williamson D, Higuchi R, Symmans WA, Brennan SO. George PM, et al. Among authors: higuchi r. Br J Haematol. 1992 May;81(1):93-8. doi: 10.1111/j.1365-2141.1992.tb08178.x. Br J Haematol. 1992. PMID: 1520631
Single-tube genotyping without oligonucleotide probes.
Germer S, Higuchi R. Germer S, et al. Among authors: higuchi r. Genome Res. 1999 Jan;9(1):72-8. Genome Res. 1999. PMID: 9927486 Free PMC article.
High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.
Germer S, Holland MJ, Higuchi R. Germer S, et al. Among authors: higuchi r. Genome Res. 2000 Feb;10(2):258-66. doi: 10.1101/gr.10.2.258. Genome Res. 2000. PMID: 10673283 Free PMC article.
The accuracy of measuring known allele frequencies is very high, with the strength of correlation between measured and known frequencies having an r(2) = 0.997. ...
The accuracy of measuring known allele frequencies is very high, with the strength of correlation between measured and known frequencies hav …
Effective amplification of long targets from cloned inserts and human genomic DNA.
Cheng S, Fockler C, Barnes WM, Higuchi R. Cheng S, et al. Among authors: higuchi r. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9. doi: 10.1073/pnas.91.12.5695. Proc Natl Acad Sci U S A. 1994. PMID: 8202550 Free PMC article.
High-resolution, high-throughput HLA genotyping by next-generation sequencing.
Bentley G, Higuchi R, Hoglund B, Goodridge D, Sayer D, Trachtenberg EA, Erlich HA. Bentley G, et al. Among authors: higuchi r. Tissue Antigens. 2009 Nov;74(5):393-403. doi: 10.1111/j.1399-0039.2009.01345.x. Tissue Antigens. 2009. PMID: 19845894 Free PMC article.
Avoiding false positives with PCR.
Kwok S, Higuchi R. Kwok S, et al. Among authors: higuchi r. Nature. 1989 May 18;339(6221):237-8. doi: 10.1038/339237a0. Nature. 1989. PMID: 2716852
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