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Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.
Higuchi R, Fockler C, Dollinger G, Watson R. Higuchi R, et al. Biotechnology (N Y). 1993 Sep;11(9):1026-30. doi: 10.1038/nbt0993-1026. Biotechnology (N Y). 1993. PMID: 7764001
Quantitation of residual WBCs in filtered blood components by high-throughput, real-time kinetic PCR.
Lee TH, Wen L, Chrebtow V, Higuchi R, Watson RM, Sninsky JJ, Busch MP. Lee TH, et al. Transfusion. 2002 Jan;42(1):87-93. doi: 10.1046/j.1537-2995.2002.00009.x. Transfusion. 2002. PMID: 11896318
The scatter graph of the 250 samples tested in duplicate by kPCR generated a slope of 1.0122 and an R(2) value of 0.9265. The comparison of kPCR and (32)P-probe hybridization results on 359 clinical samples gave a scatter-graph slope of 0.9428 and an R(2) value of 0 …
The scatter graph of the 250 samples tested in duplicate by kPCR generated a slope of 1.0122 and an R(2) value of 0.9265. The compari …
Homogeneous allele-specific PCR in SNP genotyping.
Germer S, Higuchi R. Germer S, et al. Methods Mol Biol. 2003;212:197-214. doi: 10.1385/1-59259-327-5:197. Methods Mol Biol. 2003. PMID: 12491912 No abstract available.
High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.
Wang J, Chuang K, Ahluwalia M, Patel S, Umblas N, Mirel D, Higuchi R, Germer S. Wang J, et al. Biotechniques. 2005 Dec;39(6):885-93. doi: 10.2144/000112028. Biotechniques. 2005. PMID: 16382908
Single-tube genotyping without oligonucleotide probes.
Germer S, Higuchi R. Germer S, et al. Genome Res. 1999 Jan;9(1):72-8. Genome Res. 1999. PMID: 9927486 Free PMC article.
High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.
Germer S, Holland MJ, Higuchi R. Germer S, et al. Genome Res. 2000 Feb;10(2):258-66. doi: 10.1101/gr.10.2.258. Genome Res. 2000. PMID: 10673283 Free PMC article.
The accuracy of measuring known allele frequencies is very high, with the strength of correlation between measured and known frequencies having an r(2) = 0.997. ...
The accuracy of measuring known allele frequencies is very high, with the strength of correlation between measured and known frequencies hav …
Effective amplification of long targets from cloned inserts and human genomic DNA.
Cheng S, Fockler C, Barnes WM, Higuchi R. Cheng S, et al. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9. doi: 10.1073/pnas.91.12.5695. Proc Natl Acad Sci U S A. 1994. PMID: 8202550 Free PMC article.
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