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Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.
Almer A, Rudolph H, Hinnen A, Hörz W. Almer A, et al. EMBO J. 1986 Oct;5(10):2689-96. EMBO J. 1986. PMID: 3536481 Free PMC article.
The chromatin fine structure in the promoter region of PHO5, the structural gene for a strongly regulated acid phosphatase in yeast, was analyzed. ...These findings suggest a mechanism by which the chromatin structure participates in the functioning of a regu …
The chromatin fine structure in the promoter region of PHO5, the structural gene for a strongly regulated acid phosphatase in yeast, …
Interpathway regulation of the TRP4 gene of yeast.
Braus G, Mösch HU, Vogel K, Hinnen A, Hütter R. Braus G, et al. EMBO J. 1989 Mar;8(3):939-45. EMBO J. 1989. PMID: 2656261 Free PMC article.
PHO2 and GCN4 proteins bind in a mutually exclusive manner to the same sequence, UAS1, one of two GCN4 binding sites in the TRP4 promoter. ...
PHO2 and GCN4 proteins bind in a mutually exclusive manner to the same sequence, UAS1, one of two GCN4 binding sites in the TRP4 prom …
The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.
Rudolph H, Hinnen A. Rudolph H, et al. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1340-4. doi: 10.1073/pnas.84.5.1340. Proc Natl Acad Sci U S A. 1987. PMID: 2881299 Free PMC article.
The unlinked but coordinately regulated PHO11 promoter contains a single copy of an almost identical dyad sequence, suggesting that there is a common regulatory UASp for both genes. A TATA element is absolutely required for detectable PHO5 transcription. Spec …
The unlinked but coordinately regulated PHO11 promoter contains a single copy of an almost identical dyad sequence, suggesting that t …
Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences.
Meyhack B, Bajwa W, Rudolph H, Hinnen A. Meyhack B, et al. EMBO J. 1982;1(6):675-80. EMBO J. 1982. PMID: 6329697 Free PMC article.
We have cloned the structural genes for a regulated ( PHO5 ) and a constitutive ( PHO3 ) acid phosphatase from yeast by transformation and complementation of a yeast pho3 , pho5 double mutant. ...While the nucleotide sequences of the two coding regions are qu …
We have cloned the structural genes for a regulated ( PHO5 ) and a constitutive ( PHO3 ) acid phosphatase from yeast by transf …
PHO5 upstream sequences confer phosphate control on the constitutive PHO3 gene.
Bajwa W, Rudolph H, Hinnen A. Bajwa W, et al. Yeast. 1987 Mar;3(1):33-42. doi: 10.1002/yea.320030106. Yeast. 1987. PMID: 2849256
To identify the sequences involved in the regulation of the yeast acid phosphatase gene (PHO5) we constructed a series of hybrid promoters. ...A critical regulatory element was located between position -402 to -351 (upstream from ATG) and sequences further downstrea …
To identify the sequences involved in the regulation of the yeast acid phosphatase gene (PHO5) we constructed a series of hybrid prom …
The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions.
Vogel K, Hörz W, Hinnen A. Vogel K, et al. Mol Cell Biol. 1989 May;9(5):2050-7. doi: 10.1128/mcb.9.5.2050. Mol Cell Biol. 1989. PMID: 2664469 Free PMC article.
Gel retardation assays with the PHO2 protein revealed a binding region that lay between the two PHO4-binding sites. DNase I footprint analysis suggested a PHO2-binding site covering the region between -277 and -296....
Gel retardation assays with the PHO2 protein revealed a binding region that lay between the two PHO4-binding sites. DNase I footprint …
The yeast phosphatase system.
Vogel K, Hinnen A. Vogel K, et al. Mol Microbiol. 1990 Dec;4(12):2013-7. doi: 10.1111/j.1365-2958.1990.tb00560.x. Mol Microbiol. 1990. PMID: 1965216 Review.
Yeast cells produce a set of enzymes which are involved in the metabolism of phosphate, and include acid and alkaline phosphatases as well as permeases. ...In the past few years a considerable amount of genetic and molecular evidence has accumulated and a rat …
Yeast cells produce a set of enzymes which are involved in the metabolism of phosphate, and include acid and alkaline phosphatases as …
Hepatitis B virus antigens made in microbial cells immunise against viral infection.
Murray K, Bruce SA, Hinnen A, Wingfield P, van Erd PM, de Reus A, Schellekens H. Murray K, et al. EMBO J. 1984 Mar;3(3):645-50. EMBO J. 1984. PMID: 6370689 Free PMC article.
A 28-bp segment of the Saccharomyces cerevisiae PHO5 upstream activator sequence confers phosphate control to the CYC1-lacZ gene fusion.
Sengstag C, Hinnen A. Sengstag C, et al. Gene. 1988 Jul 30;67(2):223-8. doi: 10.1016/0378-1119(88)90399-x. Gene. 1988. PMID: 3139496
Two regions within the Saccharomyces cerevisiae PHO5 upstream activator sequence (UAS) are involved in phosphate dependent transcription activation [Rudolph and Hinnen, Proc. ...A 28-bp segment is very efficient in gene activation, and a 19-bp subsegment that …
Two regions within the Saccharomyces cerevisiae PHO5 upstream activator sequence (UAS) are involved in phosphate dependent transcription act …
Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.
Bajwa W, Meyhack B, Rudolph H, Schweingruber AM, Hinnen A. Bajwa W, et al. Nucleic Acids Res. 1984 Oct 25;12(20):7721-39. doi: 10.1093/nar/12.20.7721. Nucleic Acids Res. 1984. PMID: 6093051 Free PMC article.
Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PHO5, PHO3 (3'). The mRNA transcripts have been analysed by S1-nuclease mapping. ...The comparison of the NH2-terminal amino acid sequence deduced from the nucleotide sequence, with that of puri …
Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PHO5, PHO3 (3'). The mRNA transcripts have been analys …
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