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PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases.
Cline J, Braman JC, Hogrefe HH. Cline J, et al. Among authors: hogrefe hh. Nucleic Acids Res. 1996 Sep 15;24(18):3546-51. doi: 10.1093/nar/24.18.3546. Nucleic Acids Res. 1996. PMID: 8836181 Free PMC article.
Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance.
Arezi B, McKinney N, Hansen C, Cayouette M, Fox J, Chen K, Lapira J, Hamilton S, Hogrefe H. Arezi B, et al. Among authors: hogrefe h. Front Microbiol. 2014 Aug 14;5:408. doi: 10.3389/fmicb.2014.00408. eCollection 2014. Front Microbiol. 2014. PMID: 25177317 Free PMC article.
Novel mutations in Moloney Murine Leukemia Virus reverse transcriptase increase thermostability through tighter binding to template-primer.
Arezi B, Hogrefe H. Arezi B, et al. Among authors: hogrefe h. Nucleic Acids Res. 2009 Feb;37(2):473-81. doi: 10.1093/nar/gkn952. Epub 2008 Dec 4. Nucleic Acids Res. 2009. PMID: 19056821 Free PMC article.
Mutant of Moloney murine leukemia virus reverse transcriptase exhibits higher resistance to common RT-qPCR inhibitors.
Arezi B, McCarthy M, Hogrefe H. Arezi B, et al. Among authors: hogrefe h. Anal Biochem. 2010 May 15;400(2):301-3. doi: 10.1016/j.ab.2010.01.024. Epub 2010 Jan 25. Anal Biochem. 2010. PMID: 20100452
Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide gamma-phosphate derivative.
Hansen CJ, Wu L, Fox JD, Arezi B, Hogrefe HH. Hansen CJ, et al. Among authors: hogrefe hh. Nucleic Acids Res. 2011 Mar;39(5):1801-10. doi: 10.1093/nar/gkq1053. Epub 2010 Nov 9. Nucleic Acids Res. 2011. PMID: 21062827 Free PMC article.
Long-range PCR with a DNA polymerase fusion.
Hogrefe HH, Borns MC. Hogrefe HH, et al. Methods Mol Biol. 2011;687:17-23. doi: 10.1007/978-1-60761-944-4_2. Methods Mol Biol. 2011. PMID: 20967598
Rapid quantification of DNA libraries for next-generation sequencing.
Buehler B, Hogrefe HH, Scott G, Ravi H, Pabón-Peña C, O'Brien S, Formosa R, Happe S. Buehler B, et al. Among authors: hogrefe hh. Methods. 2010 Apr;50(4):S15-8. doi: 10.1016/j.ymeth.2010.01.004. Methods. 2010. PMID: 20215015 Review.
Escherichia coli DNA polymerase III epsilon subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT-PCR procedures.
Arezi B, Hogrefe HH. Arezi B, et al. Among authors: hogrefe hh. Anal Biochem. 2007 Jan 1;360(1):84-91. doi: 10.1016/j.ab.2006.10.009. Epub 2006 Oct 30. Anal Biochem. 2007. PMID: 17107651
Amplification efficiency of thermostable DNA polymerases.
Arezi B, Xing W, Sorge JA, Hogrefe HH. Arezi B, et al. Among authors: hogrefe hh. Anal Biochem. 2003 Oct 15;321(2):226-35. doi: 10.1016/s0003-2697(03)00465-2. Anal Biochem. 2003. PMID: 14511688
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation.
Hogrefe HH, Hansen CJ, Scott BR, Nielson KB. Hogrefe HH, et al. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):596-601. doi: 10.1073/pnas.012372799. Epub 2002 Jan 8. Proc Natl Acad Sci U S A. 2002. PMID: 11782527 Free PMC article.
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