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Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase.
Varghese JN, Hrmova M, Fincher GB. Varghese JN, et al. Among authors: hrmova m. Structure. 1999 Feb 15;7(2):179-90. doi: 10.1016/s0969-2126(99)80024-0. Structure. 1999. PMID: 10368285
High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp.
Hrmova M, Stone BA, Fincher GB. Hrmova M, et al. Glycoconj J. 2010 May;27(4):461-76. doi: 10.1007/s10719-010-9291-4. Epub 2010 May 16. Glycoconj J. 2010. PMID: 20473714
Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues.
Hrmova M, Fincher GB. Hrmova M, et al. Carbohydr Res. 2007 Sep 3;342(12-13):1613-23. doi: 10.1016/j.carres.2007.05.013. Epub 2007 May 18. Carbohydr Res. 2007. PMID: 17548065 Review.
Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.).
Hrmova M, Farkas V, Harvey AJ, Lahnstein J, Wischmann B, Kaewthai N, Ezcurra I, Teeri TT, Fincher GB. Hrmova M, et al. FEBS J. 2009 Jan;276(2):437-56. doi: 10.1111/j.1742-4658.2008.06791.x. Epub 2008 Dec 4. FEBS J. 2009. PMID: 19076217
A glimpse at regulation of nitrogen homeostasis.
Kaiser BN, Hrmova M. Kaiser BN, et al. Among authors: hrmova m. Structure. 2010 Nov 10;18(11):1395-7. doi: 10.1016/j.str.2010.10.003. Structure. 2010. PMID: 21070938
A Barley Efflux Transporter Operates in a Na+-Dependent Manner, as Revealed by a Multidisciplinary Platform.
Nagarajan Y, Rongala J, Luang S, Singh A, Shadiac N, Hayes J, Sutton T, Gilliham M, Tyerman SD, McPhee G, Voelcker NH, Mertens HD, Kirby NM, Lee JG, Yingling YG, Hrmova M. Nagarajan Y, et al. Among authors: hrmova m. Plant Cell. 2016 Jan;28(1):202-18. doi: 10.1105/tpc.15.00625. Epub 2015 Dec 15. Plant Cell. 2016. PMID: 26672067 Free PMC article.
Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site.
Streltsov VA, Luang S, Peisley A, Varghese JN, Ketudat Cairns JR, Fort S, Hijnen M, Tvaroška I, Ardá A, Jiménez-Barbero J, Alfonso-Prieto M, Rovira C, Mendoza F, Tiessler-Sala L, Sánchez-Aparicio JE, Rodríguez-Guerra J, Lluch JM, Maréchal JD, Masgrau L, Hrmova M. Streltsov VA, et al. Among authors: hrmova m. Nat Commun. 2019 May 20;10(1):2222. doi: 10.1038/s41467-019-09691-z. Nat Commun. 2019. PMID: 31110237 Free PMC article.
Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus.
Nielsen KA, Hrmova M, Nielsen JN, Forslund K, Ebert S, Olsen CE, Fincher GB, Møller BL. Nielsen KA, et al. Among authors: hrmova m. Planta. 2006 Apr;223(5):1010-23. doi: 10.1007/s00425-005-0158-z. Epub 2005 Nov 24. Planta. 2006. PMID: 16307283
Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain (1,3/1,6)-beta-D-glucans, galacto- and rhamnomannans, (1,3;1,4)-beta-D-glucans and chitin.
Pettolino F, Sasaki I, Turbic A, Wilson SM, Bacic A, Hrmova M, Fincher GB. Pettolino F, et al. Among authors: hrmova m. FEBS J. 2009 Jul;276(14):3698-709. doi: 10.1111/j.1742-4658.2009.07086.x. Epub 2009 Jun 1. FEBS J. 2009. PMID: 19496815
Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase.
Hrmova M, Burton RA, Biely P, Lahnstein J, Fincher GB. Hrmova M, et al. Biochem J. 2006 Oct 1;399(1):77-90. doi: 10.1042/BJ20060170. Biochem J. 2006. PMID: 16771710 Free PMC article.
With locust-bean galactomannan and mannopentaitol as substrates, the enzyme has K(m) constants of 0.16 mg x ml(-1) and 5.3 mM and kcat constants of 12.9 and 3.9 s(-1) respectively. ...A second barley enzyme that participates in mannan depolymerization through its ability t …
With locust-bean galactomannan and mannopentaitol as substrates, the enzyme has K(m) constants of 0.16 mg x ml(-1) and 5.3 mM and kca …
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