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105 results
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Real-time RT-PCR normalisation; strategies and considerations.
Huggett J, Dheda K, Bustin S, Zumla A. Huggett J, et al. Genes Immun. 2005 Jun;6(4):279-84. doi: 10.1038/sj.gene.6364190. Genes Immun. 2005. PMID: 15815687 Review.
Assessing the accuracy of quantitative molecular microbial profiling.
O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF. O'Sullivan DM, et al. Among authors: huggett jf. Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476. Int J Mol Sci. 2014. PMID: 25421243 Free PMC article.
Considerations for digital PCR as an accurate molecular diagnostic tool.
Huggett JF, Cowen S, Foy CA. Huggett JF, et al. Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22. Clin Chem. 2015. PMID: 25338683 Review.
Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.
Sanders R, Mason DJ, Foy CA, Huggett JF. Sanders R, et al. Among authors: huggett jf. Anal Bioanal Chem. 2014 Oct;406(26):6471-83. doi: 10.1007/s00216-014-7857-x. Epub 2014 May 25. Anal Bioanal Chem. 2014. PMID: 24858468 Free PMC article. Review.
The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.
Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA. Huggett JF, et al. Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9. Clin Chem. 2013. PMID: 23570709
Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis.
Devonshire AS, Sanders R, Wilkes TM, Taylor MS, Foy CA, Huggett JF. Devonshire AS, et al. Methods. 2013 Jan;59(1):89-100. doi: 10.1016/j.ymeth.2012.07.021. Epub 2012 Jul 24. Methods. 2013. PMID: 22841564 Review.
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.
Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, Foy CA, Scott DJ. Whale AS, et al. Among authors: huggett jf. Nucleic Acids Res. 2012 Jun;40(11):e82. doi: 10.1093/nar/gks203. Epub 2012 Feb 28. Nucleic Acids Res. 2012. PMID: 22373922 Free PMC article.
Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine.
Whale AS, Jones GM, Pavšič J, Dreo T, Redshaw N, Akyürek S, Akgöz M, Divieto C, Sassi MP, He HJ, Cole KD, Bae YK, Park SR, Deprez L, Corbisier P, Garrigou S, Taly V, Larios R, Cowen S, O'Sullivan DM, Bushell CA, Goenaga-Infante H, Foy CA, Woolford AJ, Parkes H, Huggett JF, Devonshire AS. Whale AS, et al. Among authors: huggett jf. Clin Chem. 2018 Sep;64(9):1296-1307. doi: 10.1373/clinchem.2017.285478. Epub 2018 Jun 14. Clin Chem. 2018. PMID: 29903874
Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.
Busby E, Whale AS, Ferns RB, Grant PR, Morley G, Campbell J, Foy CA, Nastouli E, Huggett JF, Garson JA. Busby E, et al. Among authors: huggett jf. Sci Rep. 2017 Apr 26;7(1):1209. doi: 10.1038/s41598-017-01221-5. Sci Rep. 2017. PMID: 28446770 Free PMC article.
Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.
Pavšič J, Devonshire A, Blejec A, Foy CA, Van Heuverswyn F, Jones GM, Schimmel H, Žel J, Huggett JF, Redshaw N, Karczmarczyk M, Mozioğlu E, Akyürek S, Akgöz M, Milavec M. Pavšič J, et al. Among authors: huggett jf. Anal Bioanal Chem. 2017 Apr;409(10):2601-2614. doi: 10.1007/s00216-017-0206-0. Epub 2017 Jan 26. Anal Bioanal Chem. 2017. PMID: 28124757 Free PMC article.
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